相关实验视频

Updated: Jun 23, 2025

Optimized LC-MS/MS Method for the High-throughput Analysis of Clinical Samples of Ivacaftor, Its Major Metabolites, and Lumacaftor in Biological Fluids of Cystic Fibrosis Patients

Optimized LC-MS/MS Method for the High-throughput Analysis of Clinical Samples of Ivacaftor, Its Major Metabolites, and Lumacaftor in Biological Fluids of Cystic Fibrosis Patients

Published on: October 15, 2017

关于用于favipiravir生物分析的液体染色学方法可持续性的见解:一项比较研究

Ahmed Mostafa¹

¹Department of Pharmaceutical Chemistry, College of Clinical Pharmacy, Imam Abdulrahman Bin Faisal University King Faisal Road, P.O. Box 1982 Dammam 31441 Eastern Province Saudi Arabia ammostafa@iau.edu.sa ammostaf@uwaterloo.ca (+966) 56 262 3776.

|June 20, 2024

概括

本研究评估了19种用于favipiravir生物分析的液体染色学方法对环境的影响. 它倡导将绿色评价纳入可持续制药分析方法验证中.

科学领域:

分析化学分析化学
绿色化学绿色化学
药品分析药品分析

背景情况:

法维皮拉维尔是一种广泛的抗病毒药物,在流感和COVID-19治疗中具有应用.
有效的分析方法对于在生物样本中定量法维皮拉维尔至关重要.
传统的分析方法引发了环境和健康问题,需要更绿色的替代方案.

研究的目的:

调查19种用于favipiravir生物分析的液体染色学方法对环境的影响.
用既有评估工具评估这些分析方法的绿色和白色.
促进环境友好和经济的分析方法的发展,用于法维皮拉维尔的确定.

主要方法:

用了19种液体染色学方法进行favipiravir生物分析.
应用绿色评估工具:分析生态尺度 (AES),绿色分析程序指数 (GAPI) 和分析绿色计算器 (AGREE).
使用红绿蓝12 (RGB 12) 工具进行全球白度评估.

主要成果:

识别和评估各种favipiravir生物分析方法对环境的影响.
提供了分析方法的绿色和白色概况的见解.
证明了AES,GAPI,AGREE和RGB 12在评估分析方法可持续性的有用性.

结论:

强调在分析方法开发中需要积极规划环境影响和可持续性.
强烈倡导将绿色和白色评价纳入方法验证协议.
强调全面的全球评估对于开发高效,环保和经济的分析方法的重要性.

更多相关视频

$Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems$ 14:42

Liquid Chromatography Coupled to Refractive Index or Mass Spectrometric Detection for Metabolite Profiling in Lysate-based Cell-free Systems

Published on: September 23, 2021

Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis

Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis

Published on: July 11, 2014

相关概念视频

High-Performance Liquid Chromatography: Introduction

High-Performance Liquid Chromatography: Introduction

High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
In HPLC, two phases play a critical role in the separation process:

High-Performance Liquid Chromatography: Instrumentation

High-Performance Liquid Chromatography: Instrumentation

High-performance liquid chromatography, or HPLC, is an analytical technique that separates liquid samples under high pressures. An HPLC instrument consists of glass bottles for storing solvents called mobile phase reservoirs. HPLC-grade solvents are used to maintain high purity, and the dissolved gases are removed using a degasser, such as a vacuum pumping system or sparging with helium. The solvents are then pumped into the analytical column using a screw-driven syringe or reciprocating pumps.

High-Performance Liquid Chromatography: Elution Process

High-Performance Liquid Chromatography: Elution Process

In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...