NbPTR1 赋予了对 Pseudomonas syringae pv. 的耐药性. 在果中的actinidiae
Shin-Mei Yeh1, Minsoo Yoon2, Sidney Scott1
1New Cultivar Innovation, The New Zealand Institute for Plant & Food Research Limited (PFR), Mt Albert Research Centre, Auckland, New Zealand.
Plant, cell & environment
|June 20, 2024
概括
科学家们确定了NbPTR1作为抵抗Pseudomonas syringae pv.的关键基因. 在果中发现的actinidiae biovar 3 (Psa3). 将这种基因改造为易受感染的果品种,为打击毁灭性的瘤病提供了一个有希望的策略.
科学领域:
- 植物病理学 植物病理学
- 分子植物-微生物相互作用
- 遗传学 遗传学是一种遗传学.
背景情况:
- 伪虫的注射器 pv. 动生物菌3 (Psa3) 在果 (Actinidia chinensis) 中引起严重的癌症.
- Psa3效应因子HopZ5对于疾病爆发至关重要,但受敏感的果品种无法识别.
- 尼科蒂亚娜本塔米亚纳表现出对HopZ5的非宿主耐药性,这表明特定植物免疫基因的参与.
研究的目的:
- 为了识别尼科蒂亚娜本塔米亚纳中的基因,这些基因对Psa3效应器HopZ5.5产生抗性.
- 为了研究在敏感的Actinidia chinensis品种中发现的抗性基因的作用.
- 探索工程基维果对Psa3耐药性的潜力.
主要方法:
- 查尼科蒂亚娜本塔米亚纳中的HopZ5识别,以确定耐药性基因.
- 调查NbPTR1,RPM1相互作用蛋白4和HopZ5.5之间的蛋白质相互作用.
- 变形易感的中国菌种 (Actinidia chinensis var.) 的变形. chinensis"Hort16A"与NbPTR1一起用于评估耐药性.
主要成果:
- 核酸结合的氨酸丰富的重复受体NbPTR1被确定为N. benthamiana中的HopZ5识别因子.
- 霍普Z5破坏了NbPTR1和RPM1相互作用蛋白4 ортолог之间的复杂形成.
- 敏感的"Hort16A"桃中的NbPTR1表达赋予了对Psa3.3的HopZ5特异性耐药性.
结论:
- NbPTR1在识别Psa3效应器HopZ5中发挥着关键作用,在N. benthamiana中调解非宿主抵抗.
- 在A. chinensis中缺乏功能性的NbPTR1正基因,这有助于基维果的易感性.
- 工程基维果与NbPTR1为开发对Psa3的抵抗力和减轻瘤疾病提供了可行的策略.
关键词:
在中国的Actinidia chinensis.在HopZ5中使用HopZ5尼科蒂亚娜·本塔米亚娜 (Nicotiana benthamiana) 是一种植物.在Psa3中使用Psa3.在RNAi头发针图书馆.效应器触发的免疫力更多相关视频
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