相关实验视频

Updated: Jun 23, 2025

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Published on: April 7, 2014

紧型林尼克型高光谱定量相显微镜用于细胞组件的高级分类

Himanshu Joshi¹, Bhanu Pratap Singh¹, Ankit Butola²

¹Bio-photonics and Green-photonics Laboratory, Department of Physics, Indian Institute of Technology Delhi, New Delhi, India.

Journal of biophotonics

|June 20, 2024

概括

一个新的紧型高光谱定量相显微镜 (HS-QPM) 系统简化了复杂的技术. 这种先进的HS-QPM能够对细胞组件进行详细的光谱分析,以改善生物医学研究.

科学领域:

生物医学光学生物医学光学
显微镜的使用方法
频谱学是一种光谱学.

背景情况:

超光谱定量相显微镜 (HS-QPM) 提供了对生物样品的波长依赖分析.
传统的HS-QPM系统可能是复杂和不稳定的,限制了它们的广泛应用.
先进的细胞表征需要使用光谱信息的高分辨率成像技术.

研究的目的:

开发一个紧而稳定的林尼克型高光谱定量相显微镜 (HS-QPM) 系统.
证明系统能够从生物和工业样本中获取和分析光谱相位信息.
探索HS-QPM在生物医学研究中增强细胞表征的潜力.

主要方法:

开发一个紧的林尼克型HS-QPM系统,使用单个对象镜头,用于样品和参考臂.
从各种波长的剥皮的脸细胞中获取超光谱相位图,形成一个超光谱相位立方体.
主成分分析 (PCA) 的应用,以分析细胞组件的波长依赖反应,并确定光谱特征.

主要成果:

成功开发了一个紧而稳定的HS-QPM系统.
为生物样本生成超光谱相位立方体,揭示相位分布中的光谱维度.
通过PCA识别细胞组件中占主导地位的光谱特征,突出波长特定的光学特性.

结论:

开发的紧型HS-QPM系统有效地减少了与传统方法相关的复杂性和不稳定性.
HS-QPM为详细的细胞特征提供了有价值的光谱信息.
这种技术显示出通过增强细胞分析来推进生物医学研究和临床诊断的重大前景.

关键词:

细胞组件分类细胞组件分类超光谱定量相位显微镜.没有标签的光学技术.定量阶段显微镜学的定量阶段显微镜.

更多相关视频

Label-Free Identification of Lymphocyte Subtypes Using Three-Dimensional Quantitative Phase Imaging and Machine Learning

Label-Free Identification of Lymphocyte Subtypes Using Three-Dimensional Quantitative Phase Imaging and Machine Learning

Published on: November 19, 2018

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals

Published on: August 22, 2019

相关概念视频

Phase Contrast and Differential Interference Contrast Microscopy

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...