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Updated: Jun 23, 2025

Visualizing Protein Kinase A Activity In Head-fixed Behaving Mice Using In Vivo Two-photon Fluorescence Lifetime Imaging Microscopy
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多重复合体体内成像与光寿命调节标签

Lina El Hajji1, France Lam2, Maria Avtodeeva1

  • 1Sorbonne Université, École Normale Supérieure, Université PSL, CNRS, Laboratoire des Biomolécules, LBM, Paris, 75005, France.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)
|June 20, 2024
PubMed
概括

研究人员开发了新的光激活和吸收转移标签 (FAST) 来调节光寿命. 这使得在活细胞和生物体中使用单个光探针对多个目标进行高度多重成像.

关键词:
生物成像是一种生物成像.光终身成像成像 光终身成像光探测器的光探测器多重成像多重成像成像蛋白质工程工程 蛋白质工程

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相关概念视频

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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