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Updated: Aug 13, 2026

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细胞溶解体中的mRNA显示使得针对BRD3外终域的循环的识别成为可能

Catherine A Hurd1,2, Jacob T Bush3, Andrew J Powell3

  • 1Department of Chemistry, Imperial College London, Molecular Sciences Research Hub, London, W12 0BZ.

Angewandte Chemie (International ed. in English)
|June 20, 2024
PubMed
概括

这项研究引入了一种新的mRNA显示方法,用于选细胞溶解体中的循环,扩大药物发现目标. 这种方法成功地确定了对挑战性蛋白质 (如BRD3.3) 的高亲和性结体.

关键词:
化学工具是化学工具.药物发现 药物发现表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.酸是一种酸.在mRNA显示屏上显示mRNA.

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Nuclear Export of mRNA

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Nonsense-mediated mRNA Decay

The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
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Regulated mRNA Transport

In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing specific...
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Leaky Scanning

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mRNA Stability and Gene Expression

The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
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Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
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