揭示黑果上的真菌多样性和相关的二次代谢
Michael S Cowled1, Christopher B W Phippen1, Kresten J K Kromphardt1
1Department of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby, Denmark.
Applied and environmental microbiology
|June 20, 2024
概括
黑果含有大量的微生物二次代谢产物 (SMs),其中大多数化合物以前是未知的. 这项研究探讨了它们的化学生态及其在微生物相互作用中的潜在作用.
科学领域:
- 微生物学和生物化学
- 代谢学和自然产品化学 化学
背景情况:
- 黑果是微生物分解的结果,像*Monilinia fructigena*和*Penicillium expansum*这样的真菌是常见的入侵者.
- 关于果微生物组的化学数据有限,需要对其二次代谢物 (SM) 多样性和生态功能进行研究.
研究的目的:
- 研究黑果中二次代谢物 (SMs) 的化学多样性和潜在的生态作用.
- 识别由真菌衍生的SMs并了解它们对黑果微生物群的贡献.
主要方法:
- 对38个黑果和种植的真菌菌株进行了代谢分析 (HPLC-HRMS/MS).
- 一种一种菌株多种化合物 (OSMAC) 方法,在9个基板上分离了15种真菌,有助于SM注释.
- 针对性和非针对性代谢学被用来分析代谢产物,微生物和果宿主之间的相互作用.
主要成果:
- 确定了3319种独特的化学特征,只有6.4%匹配了光谱库中已知的化合物.
- 根据OSMAC数据,32%的黑果中检测到的特征被归类为由真菌衍生的.
- 检测到了抗真菌化合物,这表明它在果微生物群中的微生物竞争中发挥了作用.
结论:
- 与其他环境微生物相比,黑果的微生物SM多样性和丰富性明显更高.
- 已知SMs的低发病率凸显了这种系统中产生的化合物的新性.
- 进一步研究这些新型微生物的生态作用对于理解微生物相互作用和潜在应用至关重要.
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