精液微生物组疾病和精液DNA损伤之间的关联
Junxian He1,2,3,4, Menghui Ma1,2,3,4, Zhenhan Xu1,2,3,4
1Reproductive Medicine Center, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.
Microbiology spectrum
|June 20, 2024
概括
精子微生物组,特别是*Lactobacillus iners*,与高精子DNA碎片化 (HDFI) 有关,这是男性不孕症的原因. 诸如布他诺酸这样的代谢物可能会损害精子DNA,这表明针对HDFI的微生物向疗法.
科学领域:
- 生殖生物学 生殖生物学
- 微生物组研究 微生物组研究
- 男人不孕症诊断 诊断 男人不孕症诊断
背景情况:
- 精子DNA碎片化指数 (DFI) 是男性不孕症和不良生殖结果的关键生物标志物.
- 以前的研究表明,精液微生物组合和精子DNA完整性之间存在相关性.
- 微生物组影响精子DNA损伤的精确机制在很大程度上仍未被阐明.
研究的目的:
- 研究精子微生物组及其代谢活动在高精子DNA碎片化指数 (HDFI) 中的作用.
- 为了确定与HDFI相关的特定微生物概况和代谢物.
- 探索种子微生物组作为HDFI的治疗点的潜力.
主要方法:
- 组合16S rRNA测序用于微生物分析.
- 未定位的代谢组学用于识别关键代谢物.
- 来自HDFI患者和对照者的精液样本的比较分析.
主要成果:
- 发现特定的微生物形状有助于HDFI.
- 在HDFI患者中, *Lactobacillus iners* 的含量显著增加.
- 在HDFI样本中观察到乙-CoA发酵到布他诺酸II和 purin核基降解I途径的丰富,而布他诺酸与精子DNA损伤有关.
结论:
- 精子微生物组在精子DNA碎片化中起着重要作用.
- 乳杆菌和酸盐代谢是HDFI的潜在贡献者.
- 向精液微生物组为HDFI患者提供了一种新的治疗策略.
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