相关实验视频

Updated: Jun 23, 2025

Use of Single Chain MHC Technology to Investigate Co-agonism in Human CD8+ T Cell Activation

Use of Single Chain MHC Technology to Investigate Co-agonism in Human CD8+ T Cell Activation

Published on: February 28, 2019

人类细胞巨乳病毒US10的多模式HLA-I基因型调节以及由此产生的表面图案

Carolin Gerke^1,2,3,4, Liane Bauersfeld^1,2, Ivo Schirmeister^1,2

¹Institute of Virology, Medical Center University of Freiburg, Freiburg, Germany.

|June 20, 2024

概括

人类细胞巨乳病毒 (HCMV) 糖蛋白US10独特地向人类白细胞抗原I类 (HLA-I) 分子. 这种相互作用会根据基因型对HLA-I表达产生各种影响,影响免疫反应.

科学领域:

免疫学免疫学免疫学
病毒学病毒学
分子生物学分子生物学

背景情况:

人类白细胞抗原I类 (HLA-I) 分子对于NK和T细胞对人类细胞巨核病毒 (HCMV) 的反应至关重要.
包括糖蛋白US10在内的HCMV编码的免疫酶操纵HLA-I以促进病毒传播.
通过US10影响经典HLA-I抗原呈现的精确机制尚未完全理解.

研究的目的:

为了研究HCMV US10和各种HLA-I分子之间的相互作用.
阐明US10对HLA-I表达和功能的基因型和全型特异性影响.
了解US10介导的HLA-I操纵对病毒免疫逃避的影响.

主要方法:

具有约束力的测试来评估US10与HLA-I重链 (HLA-A, -B, -C, -E, -G) 的相互作用.
对HLA-I招募到负载复合物的分析.
对HLA-I成熟和细胞表面表达的基因型特异性评估.
无偏见的HLA-I结合体分析.
对受HCMV感染的纤维细胞与抑制的US10和US11转录的研究.

主要成果:

US10与所有经过测试的HLA-I重链结合.
US10结合导致HLA-I招募受损,导致载复合体.
观察到显著的基因型和异型特异性影响:HLA-A避免了下调,HLA-B显示成熟受损,HLA-C/HLA-G被保留在内分泌网膜中.
感染细胞中US10/US11转录的抑制对HLA-A和HLA-B抗原呈现产生了差异影响.

结论:

HCMV US10对HLA-I分子表现出多模式和无与伦比的基因型特异性影响.
US10对HLA-I全型的差异向代表了一种复杂的病毒免疫逃避策略.
了解这些US10介导的效应对于制定打击HCMV感染的策略至关重要.

关键词:

在MHC I类中,MHC是I类.10美元10美元人类人类人类人类人类人类人类人类细胞巨乳病毒免疫系统中的疫苗.传染病是一种传染性疾病.微生物学的微生物.化物加载复杂的复杂.在Tapasin餐厅.病毒病毒病毒病毒.

更多相关视频

Assessing the Expression of Major Histocompatibility Complex Class I on Primary Murine Hippocampal Neurons by Flow Cytometry

Assessing the Expression of Major Histocompatibility Complex Class I on Primary Murine Hippocampal Neurons by Flow Cytometry

Published on: May 19, 2020

Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis

Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis

Published on: October 15, 2021

相关概念视频

Antigens Involved in Adaptive Immunity

Antigens Involved in Adaptive Immunity

An antigen is any substance the immune system identifies as foreign and potentially harmful to the body, prompting an immune response. Antigens have two functional properties: immunogenicity and reactivity. Immunogenicity is the ability of an antigen to stimulate a specific immune response. At the same time, reactivity describes the antigen's ability to react with the cells and antibodies produced in response to it.
Complete Antigens
Complete antigens possess both immunogenicity and...

T Cell Activation and Clonal Selection

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...

Antigen Processing Pathways

Antigen Processing Pathways

MHC molecules are key players in the immune response, enabling T cells to recognize and respond to specific antigens. They are present on the surface of all nucleated cells in the body and are instrumental in presenting antigens to T cells and activating them. T cells recognize the MHC-antigen complex and initiate an immune response. MHC class I and MHC class II are two main types of MHC molecules, each associated with a distinct antigen processing pathway.
MHC Class I: Presenting Endogenous...