在健康的马中,基于16S rRNA的皮肤细菌微生物组的组成和多样性
Viola Strompfová1, Lucia Štempelová2
1Centre of Biosciences of the Slovak Academy of Sciences, Institute of Animal Physiology, Šoltésovej 4-6, 040 01, Košice, Slovakia. strompfv@saske.sk.
Veterinary research communications
|June 20, 2024
概括
健康的马皮有各种各样的细菌,其中蛋白质细菌最为丰富. 各种物种的均性在各个身体部位上有显著的差异,部显示出最高的多样性.
科学领域:
- 兽医皮肤病学 兽医皮肤病学
- 微生物组研究 微生物组研究
- 马的健康 马的健康
背景情况:
- 了解马皮肤微生物群对于管理皮肤疾病至关重要.
- 皮肤微生物组在宿主生理学和健康方面发挥着至关重要的作用.
- 以前的研究已经强调了细菌成分对皮肤最佳功能的重要性.
研究的目的:
- 在临床上健康的雪特兰小马中描述皮肤微生物群结构.
- 在马皮上识别主导的细菌类和属.
- 为了评估不同身体区域的微生物群多样性的变化.
主要方法:
- 从30只雪特兰小马的5个身体部位 (子,背部,腹部,后背,嘴巴) 的皮肤样本进行分类学分析.
- 16S rRNA基因V3-V4区域放大用于细菌识别.
- 对物种丰富性 (Chao1) 和均性 (Shannon) 指数的分析.
主要成果:
- 马的皮肤微生物群包括18个类,29个类和119个家族.
- 蛋白质细菌 (30.8%) 是最丰富的类,其次是Actinobacteriota (20.4%) 和Firmicutes (19.5%).
- Corynebacterium (7.4%) 是最丰富的属;物种均性差异很大 (p=0.0049),部呈现最大的均性.
结论:
- 马皮是复杂多样化的细菌群落的所在地.
- 细菌组成显示不同皮肤部位均性的区域差异.
- 这种表征为未来关于马皮肤健康和疾病管理的研究提供了基线.
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