用于人类脏的多式样化分析,同时进行高通量ATAC和RNA表达的协议,并进行测序
Haikuo Li1, Benjamin D Humphreys2
1Division of Nephrology, Department of Medicine, Washington University in St. Louis, St. Louis, MO 63105, USA.
STAR protocols
|June 20, 2024
概括
这项研究引入了同时高通量ATAC和RNA表达与测序 (SHARE-seq) 用于分析人类脏. 这种具有成本效益的协议可以实现大规模的单细胞多组数据生成.
科学领域:
- 基因组学就是基因组学.
- 分子生物学分子生物学
- 生物技术是生物技术.
背景情况:
- 了解细胞功能需要整合基因表达和染色质可访问性数据.
- 当前的多体经济学方法对于大规模研究来说可能是昂贵和复杂的.
研究的目的:
- 介绍使用SHARE-seq.的人类脏多式模式分析的详细协议.
- 为了使研究人员能够有效地生成高通量单细胞多组数据.
主要方法:
- 处理固定核用于SHARE-seq分池条形码和图书馆准备.
- 优化Tn5转化酶度用于转化和标记.
- 高通量测序用于同时转录和染色体可访问性分析.
主要成果:
- 在人类细胞中用于SHARE-seq的强大协议.
- 演示生成大规模的单细胞多组数据.
- 用于多组学分析的成本效益高的试剂使用.
结论:
- SHARE-seq为人类脏的单细胞多组学研究提供了一个强大的工具.
- 该协议有助于生成全面的细胞地图.
- 这种方法降低了大规模多组学研究的障碍.
相关概念视频
RNA-seq
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Ribosome Profiling
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...


