斑马鱼的胚胎酒精暴露使成年人易患心肌病和腹功能障碍
Olivia Weeks1,2, Xinlei Gao1,2, Sandeep Basu1,2
1Division of Basic and Translational Cardiovascular Research, Department of Cardiology, Boston Children's Hospital, 300 Longwood Avenue, Boston, MA 02115, USA.
Cardiovascular research
|June 20, 2024
概括
胚胎性酒精暴露 (EAE) 在斑马鱼中引起成人发作的心肌病和腹功能障碍. 这项研究确定了与EAE诱导的心脏病相关的新型基因,为风险分层提供了潜在的生物标志物.
科学领域:
- 心血管科学 心血管科学
- 发展生物学 发展生物学
- 遗传学 遗传学是一种遗传学.
背景情况:
- 胎儿酒精谱系障碍 (FASDs) 影响全球人口的高达0.8%.
- 成年FASD患者的心血管结果和心脏风险生物标志物基本上是未知的.
- 胚胎性酒精暴露 (EAE) 是一种已知的致病原体,具有潜在的长期健康后果.
研究的目的:
- 研究EAE对成年斑马鱼心脏结构,功能和基因表达的长期影响.
- 在发育期间接触过酒精的个体中确定心脏风险分层的潜在生物标志物.
- 描述EAE,先天性心脏缺陷 (CHD) 和成人发作的心脏功能障碍之间的关系.
主要方法:
- 使用斑马鱼模型进行纵向队列研究.
- 通过心声学来量化心脏结构 (心室大小) 和功能 (心缩和心缩参数).
- 腹腔的RNA测序以识别差异表达基因 (DEG) 并将它们与心脏功能相关联.
主要成果:
- 不管心脏病的严重程度如何,EAE会导致成年斑马鱼的持续性心肌病症和透析功能障碍.
- >30%的EAE成年人表现出心房与心室比率的改变,心室填充受损,心肌放松减少.
- 通过RNA测序发现了与EAE诱导的心脏病和腹筋功能障碍相关的新基因 (例如,slc25a33,ankrd9,edn1).
结论:
- EAE是成人发病心肌病和腹筋功能障碍的重要危险因素.
- 确定了EAE诱导心脏病的新型分子指标,表明了潜在的生物标志物.
- 这项研究提供了关于产前酒精暴露的终身心血管后果的见解.
更多相关视频
08:22Quantification of Ethanol Levels in Zebrafish Embryos Using Head Space Gas Chromatography
Published on: February 11, 2020
5.6K
04:50Modeling Fetal Alcohol Spectrum Disorders in Zebrafish to Characterize the Impact of an Adverse Embryonic Environment on Adult Social Behavior
Published on: February 9, 2024
316
相关概念视频
02:57Meiosis vs. Mitosis
39.5K
Cell division is necessary for growth and reproduction in organisms. Mitosis aids cell growth and development by dividing somatic cells. In contrast, meiosis causes the division of germ cells and plays an essential role in sexual reproduction. Due to their unique functional requirements, mitosis and meiosis differ from each other in multiple aspects.
Before the start of mitosis and meiosis I, the cell synthesizes DNA, resulting in two homologous copies of each chromosome. DNA synthesis is...
Before the start of mitosis and meiosis I, the cell synthesizes DNA, resulting in two homologous copies of each chromosome. DNA synthesis is...
39.5K
01:07Teratogenicity
4.2K
The ability of a drug to produce structural deformations and functional abnormalities in the developing embryo or the fetus is called teratogenicity, and the drug producing this effect is known as a teratogen. Teratogenic effects include stillbirth, miscarriage, intrauterine growth restriction, and neurocognitive delay. A teratogen may affect the embryo at different stages of development, which is important in determining the type and extent of the damage. During blastocyst formation, the early...
4.2K