调节Ce原子位点的第二协调和载体的重塑使单原子纳米酶能够有效地表达类似氧化酶的活动
1College of Chemistry, Chemical Engineering and Resource Utilization, Key Laboratory of Forest Plant Ecology, Northeast Forestry University, Harbin 150040, People's Republic of China.
Nano letters
|June 20, 2024
概括
这项研究通过将硫嵌入到原子的第二协调中来增强单原子纳米酶 (SAN). 这种修饰增强了它们的类似酶的活性,用于诸如基胆化酶测定等应用.
科学领域:
- 材料科学 材料科学 材料科学
- 纳米技术 纳米技术
- 催化剂是一种催化剂.
背景情况:
- 单原子纳米酶 (SAN) 提供高原子利用,模仿自然酶.
- 提高SAN的活动和稳定性对于其实际应用至关重要.
- 控制局部协调环境和支持结构是提高纳米酶性能的关键.
研究的目的:
- 开发一种策略来操纵SAN中的 (Ce) 原子的第二个协调.
- 重塑碳载体以改善SANS的氧化酶类活性.
- 为了证明工程SANs在生物传感测试中的应用.
主要方法:
- 在第二个协调 (Ce-N4S2-C结构) 中嵌入硫 (S) 原子的合成基于Ce的SAN.
- 用于在现场聚合多多巴胺用于前体制备和金属离子捕获.
- 使用聚乙烯甘醇 (PEG) 来调节材料接口,以提高分散和质量转移.
- 评估了合成的SANs的氧化酶类活性.
主要成果:
- Ce-N4S2-C结构有效地降低了O2减少的能量屏障,并促进了电子转移.
- 嵌入S原子增强了Ce和O原子之间的相互作用,促进了催化活性.
- 聚多巴胺涂层促进了金属来源的捕获,并在热解过程中保存了载体结构.
- PEG修改改善了SAN的水分散性和质量转移效率.
- 开发的PEG@P@Ce-N/S-C纳米酶在一个多式试验中显示出Butyrylcholinesterase活性的有效性.
结论:
- 操纵第二协调和载体结构的策略显著增强了SAN氧化酶类活性.
- Ce-N4S2-C结构是高性能单原子纳米酶的一个有希望的设计.
- 设计的SAN展示了敏感和高效的生物传感应用的潜力.
相关概念视频
Electron Carriers
84.3K
Electron carriers can be thought of as electron shuttles. These compounds can easily accept electrons (i.e., be reduced) or lose them (i.e., be oxidized). They play an essential role in energy production because cellular respiration is contingent on the flow of electrons.
Over the many stages of cellular respiration, glucose breaks down into carbon dioxide and water. Electron carriers pick up electrons lost by glucose in these reactions, temporarily storing and releasing them into the electron...
Over the many stages of cellular respiration, glucose breaks down into carbon dioxide and water. Electron carriers pick up electrons lost by glucose in these reactions, temporarily storing and releasing them into the electron...
84.3K
Electron Transport Chain: Complex III and IV
7.4K
During the electron transport chain, electrons from NADH and FADH2 are first transferred to complexes I and II, respectively. These two complexes then transfer the electrons to ubiquinol, which carries them further to complex III. Complex III passes the electrons across the intermembrane space to Cyt c, which carries them further to complex IV. Complex IV donates electrons to oxygen and reduces it to water. As electrons pass through complexes I, III, and IV, the energy released aids the pumping...
7.4K
The Supercomplexes in the Crista Membrane
2.5K
The mitochondrial cristae membrane is the primary site for the oxidative phosphorylation (OXPHOS) process of energy conversion mediated through respiratory complexes I to V. These complexes have been widely studied for decades, and it has been proven that they form supramolecular structures called respiratory supercomplexes (SC). These higher-order complexes may be crucial in maintaining the biochemical structure and improving the physiological activity of the individual complexes while...
2.5K
Oxidation and Reduction of Organic Molecules
6.4K
Energy production within a cell involves many coordinated chemical pathways. Most of these pathways are combinations of oxidation and reduction reactions, which occur at the same time. An oxidation reaction strips an electron from an atom in a compound, and the addition of this electron to another compound is a reduction reaction. Because oxidation and reduction usually occur together, these pairs of reactions are called redox reactions.
The removal of an electron from a molecule, results in a...
The removal of an electron from a molecule, results in a...
6.4K
Introduction to Mechanisms of Enzyme Catalysis
8.1K
For many years, scientists thought that enzyme-substrate binding took place in a simple "lock-and-key" fashion. This model stated that the enzyme and substrate fit together perfectly in one instantaneous step. However, current research supports a more refined view scientists call induced fit. The induced-fit model expands upon the lock-and-key model by describing a more dynamic interaction between enzyme and substrate. As the enzyme and substrate come together, their interaction causes...
8.1K
Electron Transport Chain: Complex I and II
12.9K
The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...
ROS generation is regulated and maintained at moderate levels necessary...
12.9K
![Protein Film Infrared Electrochemistry Demonstrated for Study of H2 Oxidation by a [NiFe] Hydrogenase](/_next/image?url=https%3A%2F%2Fcloudfront.jove.com%2FCDNSource%2Fteasers%2F55858.jpg&w=3840&q=50)

