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来自第二季度人类羊水的细胞外囊泡作为对膜输送器,抵消氧化应激

Giorgia Senesi¹, Laura Guerricchio², Maddalena Ghelardoni³

¹Cardiovascular Theranostics, Istituto Cardiocentro Ticino and Laboratories for Traslational Research Ente Ospedaliero Cantonale, CH-6500, Bellinzona, Switzerland; Euler Institute, Faculty of Biomedical Sciences, Università della Svizzera Italiana, CH-6900, Lugano, Switzerland.

|June 20, 2024

概括

来自人胎液 (hAF) 的细胞外囊泡 (EVs) 能够防止氧化应激和肌肉损伤. 这些AhaF衍生的EV为再生医学应用提供了有前途的治疗选择.

科学领域:

再生医学是一种再生医学.
细胞生物学细胞生物学
生物化学生物化学

背景情况:

人类羊水 (hAF) 含有具有副潜在的 stromal progenitors (hAFSC).
来自AhaFSC的细胞外囊泡 (EV) 在临床前模型中显示治疗效果.
从 hAF直接调查EV提供了细胞培养衍生EV的替代方案.

研究的目的:

为了对来自AHAF的直接缩的EV进行分析.
调查aHF-EVs对抗氧化应激的近潜力.
评估AHAF-EVs作为肌肉损伤的治疗来源.

主要方法:

处理了aHF样本以隔离和缩EVs.
使用纳米粒子跟踪分析,电子显微镜,西布洛特和流动细胞计学来表征EV.
蛋白质组学和RNA测序分析了EV货物;在氧化应激的体外模型中测试了对膜潜力.

主要成果:

获得了每毫升 hAF ~6.3x10^9 EVs 的产量,具有特有的形态和表面标记 (CD81,CD63,CD9).
hAF-EVs表达了CD133/1,CD24,CD29和SSEA4等标记物,并表现出ATP的产生.
hAF-EVs在氧化应激下挽救了C2C12细胞活力,减少了损伤和脂质过氧化,改善了心脏微组织的存活率和减少了纤维化.

结论:

剩余的II季度 hAF是电动汽车的可行来源.
F-EVs可以抵消目标细胞的氧化损伤.
hAF-EVs代表了骨和心脏肌肉损伤的新疗法.

关键词:

胚胎液是什么胚胎液是什么胚胎液是什么细胞活力细胞活力细胞外囊泡中的细胞外囊泡.代谢功能障碍代谢功能障碍氧化应激是一种氧化应激.帕拉克林效应的作用

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