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慢性炎症降低了雄羊的弧形kisspeptin表达

A N Renwick¹, B K Whitlock¹, C C Nestor²

¹Large Animal Clinical Sciences Department, University of Tennessee College of Veterinary Medicine, Knoxville, TN.

Domestic animal endocrinology

|June 20, 2024

概括

由脂聚糖 (LPS) 引起的慢性炎症显著降低了雄羊的黄素化激素 (LH) 分泌和kisspeptin神经元. 这项研究模拟了LPS如何影响牲畜的生殖轴,突出了炎症.

科学领域:

生殖内分泌学生殖内分泌学
神经免疫学神经免疫学
畜牧科学畜牧科学畜牧科学

背景情况:

来自格拉姆阴性细菌的脂聚糖 (LPS) 通过抑制性腺激素释放激素 (GnRH) 和黄素化激素 (LH) 来触发免疫反应和生殖功能受损.
虽然急性炎症对kisspeptin的影响是已知的,但慢性炎症对牲畜中这种生殖神经的影响仍然不清楚.

研究的目的:

调查LPS抑制绵羊LH分泌的中心机制.
为了检查不同的LPS施用方案 (急性,每日,增长,慢性) 对生殖激素分泌和kisspeptin神经元活动的影响.

主要方法:

20名患者被分为五组:对照组,一次性急性LPS,每日急性LPS,每日增加剂量的LPS和慢性皮下LPS.
在第1天和第7天收集血液样本以测量LH脉冲频率.
脑下垂体组织进行了免疫组织化学处理,以量化kispeptin免疫阳性细胞.

主要成果:

在第一天,所有LPS治疗组的LH脉冲频率与对照组相比显著降低.
在第7天,只有每日增加剂量的LPS组表现出明显降低的LH脉冲频率.
与对照组相比,每日增加剂量的LPS组也显示了kispeptin免疫阳性细胞的显著减少.

结论:

每天增加的LPS剂量有效地抑制年轻雄羊的kisspeptin神经元.
这种模型对于研究慢性炎症对牲畜生殖轴的影响具有重要意义.
炎症显著破坏羊的生殖神经内分泌功能.

关键词:

炎症炎症是一种炎症.亲吻peptin 的时间脂聚糖类糖化物脂聚糖类糖化物流化激素的作用是什么?绵羊是一种羊.

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