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Updated: Jun 23, 2025

A Mouse Model to Investigate the Role of Cancer-Associated Fibroblasts in Tumor Growth
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纤维肌菌素和维特龙菌素通过AKT/MDM2/P53通路在长期培养期间减轻脂肪系干细胞衰老.

Patcharapa Tragoonlugkana1, Chatchai Pruksapong2, Pawared Ontong3

  • 1Department of Clinical Microscopy, Faculty of Medical Technology, Mahidol University, 999 Phutthamonthon Sai 4, Salaya, Phutthamonthon, Nakhon Pathom, 73170, Thailand.

Scientific reports
|June 20, 2024
PubMed
概括

纤维菌素 (FN) 和维特龙菌素 (VN) 表面涂层可以减缓脂肪衍生干细胞 (ADSC) 中的细胞衰老. 这提高了再生医学和细胞疗法的细胞质量.

关键词:
脂肪酸衍生干细胞的干细胞.细胞培养培养的细胞培养.细胞疗法是一种细胞疗法.纤维纤维素的生长方式良好的制造实践是指良好的制造实践.复制性衰老是一种复制性衰老.维特罗尼克丁 (Vitronectin) 是一种葡萄素.

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科学领域:

  • 细胞生物学 细胞生物学
  • 再生医学是一种再生医学.
  • 生物材料科学 生物材料科学

背景情况:

  • 细胞衰老有助于与年龄相关的退行性疾病.
  • 二维细胞培养诱导复制性衰老,影响细胞治疗的潜力.
  • 脂肪基衍生干细胞 (ADSC) 对于细胞疗法至关重要,但在扩张过程中容易衰老.

研究的目的:

  • 为了研究纤维内素 (FN) 和视内素 (VN) 表面修饰在长期扩张期间对ADSC特征的影响.
  • 评估这些涂层对细胞衰老,粘附和增殖的影响.

主要方法:

  • 长期扩张的ADSCs培养在表面上涂有FN和VN.
  • 细胞粘附,增殖和衰老标志物的评估 (SA-β-gal活性,p16,p21,p53基因表达).
  • 对整合素信号通路 (PI3K/AKT) 和MDM2-p53相互作用的分析.

主要成果:

  • FN和VN涂层显著增强了ADSC的粘附和扩散.
  • 这些涂层减缓了细胞衰老,由SA-β-gal活性降低和衰老标记物表达率降低 (p16,p21,p53) 证明.
  • 观察到整合素 (α5,αv) 和AKT信号的升级,导致MDM2-介导的p53降解. 抑制MDM2会增加衰老和炎症.

结论:

  • 在扩大的ADSC中,FN和VN表面修饰有效地减轻了细胞衰老.
  • 这一策略通过增强增殖和延迟衰老来提高基于细胞的治疗的ADSC质量.
  • 了解这些表面细胞相互作用为优化ADSC培养用于治疗应用提供了实用方法.