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进行比较的转录组分析显示,紫色卜 (Raphanus sativus L.) 中的安托素生物合成的转录调节

Yi Liu¹, Chenchen Wang¹, Haidong Chen¹

¹College of Life Sciences, Ganzhou Key Laboratory of Greenhouse Vegetable, Gannan Normal University, Ganzhou, China.

|June 20, 2024

概括

卜的颜色变化是复杂的. 这项研究确定了关键基因RSDFR.9c和RSUGT78D2.2c,它们调节了卜芽,叶子,根和花中的安托素生物合成.

科学领域:

植物生物学植物生物学
分子遗传学分子遗传学
生物化学生物化学

背景情况:

卜 (Raphanus sativus) 在各种组织中表现出多样化的色彩,包括芽,叶,根和花.
虽然注意到了颜色的变化,但控制卜花颜色和其他组织的特定分子机制在很大程度上仍未被阐明.
现有的研究缺乏对跨多种卜组织的安东氨酸生物合成变异机制的全面调查.

研究的目的:

系统地分析四种卜组织 (芽,叶子,有肉的根和花) 中的安东素生物合成的调节机制.
为了确定关键的基因和途径,涉及到卜中安东素的差异积累.
为了阐明卜多种颜色的分子基础.

主要方法:

白色和紫色卜花的转录基因组测序.
整合已发表的转录组数据,用于卜芽,叶子和肉质根.
进行比较转录组分析,以识别在氨酸生物合成途径中差异表达的基因.
权重基因共同表达网络分析 (WGCNA) 以确定枢纽基因.

主要成果:

在四种卜组织中观察到关键的素生物合成途径基因 (DFR,UGT78D2,TT12,CPC) 的差异表达.
WGCNA确定了rsDFR.9c和rsUGT78D2.2c作为重要的枢纽基因,调节了氨酸生物合成.
发现rsPAP1副本的组织特异性表达模式对安托西亚宁合成和积累至关重要.

结论:

建议RsDFR.9c和RsUGT78D2.2c作为多种卜组织中氨酸生物合成的关键调节剂.
这项研究提供了对控制卜中安东素积累的分子机制的新见解.
了解这些调节网络可以帮助培育具有所需颜色特征的卜.

关键词:

安东基亚氨基酸是一种安东基亚氨基酸.在 DFR 中,DFR 是一个字符串.PAP1 是一个APP1.卜卜卜是什么意思卜卜卜是什么意思转录组转录组就是一个转录组.

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