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Updated: Jun 23, 2025

Isolation of Region-specific Microglia from One Adult Mouse Brain Hemisphere for Deep Single-cell RNA Sequencing

Isolation of Region-specific Microglia from One Adult Mouse Brain Hemisphere for Deep Single-cell RNA Sequencing

Published on: December 3, 2019

长读RNA测序识别了特定区域和性别的C57BL/6J小鼠大脑mRNA异型表达和使用

Emma F Jones¹, Timothy C Howton¹, Victoria L Flanary¹

¹Department of Cell, Developmental and Integrative Biology, Heersink School of Medicine, The University of Alabama at Birmingham, Birmingham, AL, United States of America.

Molecular brain

|June 20, 2024

概括

替代拼接 (AS) 在老鼠大脑区域和性别之间有所不同. 长读测序揭示了显著的差异,特别是在小脑和皮质,为神经和精神疾病提供了洞察力.

科学领域:

神经科学是一个神经科学.
基因组学就是基因组学.
分子生物学分子生物学

背景情况:

替代拼接 (AS) 推动了跨物种,性别和组织的生物多样性.
AS的失调与许多疾病有关,使其准确的测量对理解分子表型至关重要.
长读数测序 (LRS) 与短读数方法相比,为量化差异化异形态表达提供了更高的准确性.

研究的目的:

为了研究小鼠大脑内的替代拼接的性别和区域特异性差异.
为了利用长读RNA测序 (lrRNA-Seq) 对转录组变异进行全面分析.
为研究界提供一个有价值的数据资源和分析工具.

主要方法:

牛津纳米孔技术 (ONT) 生成和分析了来自两性四个不同的小鼠大脑区域 (小脑,皮质,海马,条纹体) 的长读RNA测序 (lrRNA-Seq) 数据.
量化差异基因表达 (DGE),差异转录表达 (DTE) 和差异转录使用 (DTU) 跨区域和性别.
开发了一个Shiny网络应用程序,用于交互式数据探索.

主要成果:

在所有分析的大脑区域中确定了显著的DGE,DTE和DTU,小脑表现出最多的变化.
观察到区别拼接中的特定区域性别差异,最显著的是皮质内的DTU;海马体没有显示出任何显著的基于性别的DTU.
描述了两种性别偏见的DTU模式:性别分离和性别特异.

结论:

替代拼接在区域和性别之间的大脑异质性中发挥着重要作用.
长读序列是剖析大脑中复杂的替代拼接模式的强大工具.
在拼接中发现的性别差异可能有助于理解神经和精神疾病中的性别差异.

关键词:

脑子脑子脑子脑子异形的使用异形的使用长时间读取RNA测序.性爱性的性爱性的性爱转录使用情况转录使用情况

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