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The Tumor Microenvironment

The Tumor Microenvironment

Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...

Skin Cancer

Skin Cancer

Skin cancer is a type of cancer that occurs when there is an abnormal growth of skin cells, usually triggered by damage to the DNA within the skin cells. It is primarily caused by exposure to ultraviolet (UV) radiation from the sun or artificial sources like tanning beds. Skin cancer is the most common type of cancer worldwide, and its incidence continues to rise.
Basal Cell Carcinoma (BCC): BCC is the most common type of skin cancer, accounting for about 80% of cases. It typically develops in...

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Updated: Apr 14, 2026

Tissue Engineering of Tumor Stromal Microenvironment with Application to Cancer Cell Invasion

Tissue Engineering of Tumor Stromal Microenvironment with Application to Cancer Cell Invasion

Published on: March 18, 2014

通过使用动态细胞矩阵球体组合的工程瘤肌体形态变异.

Michael J Buckenmeyer¹, Elizabeth A Brooks¹, Madison S Taylor¹

¹Cancer Biomaterials Engineering Laboratory, Cancer Innovation Laboratory, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Frederick, MD, 21702, USA.

bioRxiv : the preprint server for biology

|June 21, 2024

概括

此摘要是机器生成的。

研究人员开发了一种新的3D MatriSpheres系统,模仿瘤微环境. 这种工程外细胞矩阵 (ECM) 允许瘤细胞自我组织,影响恶性瘤并帮助精确医学.

关键词:

在体外3D建模.生物材料是一种生物材料.脱细胞化脱细胞化细胞外矩阵是细胞外矩阵.球形状的球形体是指球形状的球体.瘤微环境是一个微环境.

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A Novel Stromal Fibroblast-Modulated 3D Tumor Spheroid Model for Studying Tumor-Stroma Interaction and Drug Discovery

A Novel Stromal Fibroblast-Modulated 3D Tumor Spheroid Model for Studying Tumor-Stroma Interaction and Drug Discovery

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Heteromulticellular Stromal Cells in Scaffold-free 3D Cultures of Epithelial Cancer Cells to Drive Invasion

Heteromulticellular Stromal Cells in Scaffold-free 3D Cultures of Epithelial Cancer Cells to Drive Invasion

Published on: April 4, 2025

相关实验视频

Last Updated: Apr 14, 2026

Tissue Engineering of Tumor Stromal Microenvironment with Application to Cancer Cell Invasion

Tissue Engineering of Tumor Stromal Microenvironment with Application to Cancer Cell Invasion

Published on: March 18, 2014

A Novel Stromal Fibroblast-Modulated 3D Tumor Spheroid Model for Studying Tumor-Stroma Interaction and Drug Discovery

A Novel Stromal Fibroblast-Modulated 3D Tumor Spheroid Model for Studying Tumor-Stroma Interaction and Drug Discovery

Published on: February 28, 2020

Heteromulticellular Stromal Cells in Scaffold-free 3D Cultures of Epithelial Cancer Cells to Drive Invasion

Heteromulticellular Stromal Cells in Scaffold-free 3D Cultures of Epithelial Cancer Cells to Drive Invasion

Published on: April 4, 2025

科学领域:

生物医学工程生物医学工程
癌症生物学癌症生物学
细胞外矩阵研究研究

背景情况:

瘤微环境的复杂细胞外基质 (ECM) 对癌症的进展至关重要,但很难在体外复制.
现有的体外模型往往无法捕捉瘤细胞与其本源ECM之间的复杂相互作用.

研究的目的:

为了设计一个新的3D MatriSpheres系统,重复原生瘤微环境.
调查自组装ECM如何影响结直肠癌 (CRC) 细胞表型和恶性病变.

主要方法:

开发了一种使用脱细胞化小肠子粘膜 (SIS) ECM的自组装系统,用CRC细胞创建3D矩形球.
使用蛋白质组分析来比较SIS ECM与传统的Matrigel用于CRC同类.
采用单细胞RNA测序来分析3D模型中的瘤细胞的转录变化.

主要成果:

使用SIS ECM成功创建了3D CRC MatriSpheres,显示出与本地CRC ECM的蛋白质同质性比Matrigel更强.
CRC细胞积极组织和集中SIS ECM,在4-5天内形成富含ECM的3D流体状区域.
ECM组织和线索调节了与恶性瘤,脂质代谢和免疫调节相关的癌细胞程序,与体内发现一致.

结论:

自组装系统可以实现可扩展的,自发的工程ECM丰富的3D瘤模型.
这种方法刺激瘤特异性的组织形态发生,整合癌细胞和ECM复杂性.
3D MatriSpheres模型显示了通过更好地反映体内瘤生物学来推进精密医学的潜力.