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相关概念视频

Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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Prokaryotic Cells01:28

Prokaryotic Cells

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Prokaryotes are small unicellular organisms that include the domains — Archaea and Bacteria. Bacteria include many common microorganisms, such as Salmonella and E. coli, while the Archaea include extremophiles that live in harsh environments, such as volcanic springs.
Like eukaryotic cells, all prokaryotic cells are surrounded by a plasma membrane, have genetic material in the form of single, circular DNA, a cytoplasm that fills the interior of the cell, and ribosomes that synthesize...
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During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
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相关实验视频

Updated: Jun 23, 2025

Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins
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Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins

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神秘的细菌微型蛋白质组

Igor Fesenko1, Harutyun Sahakyan1, Svetlana A Shabalina1

  • 1National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA.

bioRxiv : the preprint server for biology
|June 21, 2024
PubMed
概括
此摘要是机器生成的。

研究人员探索了编码微蛋白的细菌小型开放读取框架 (smORF),发现了在选择中的67,297个集群. 这些微蛋白可能会调节邻近基因的活动,特别是在压力期间.

关键词:
细菌 细菌 细菌是一种细菌.生物信息学是一种生物信息学.在新的 de novo.对smORFs的进化分析.跨基因的 跨基因微型蛋白质是一种微型蛋白质.蛋白质结构预测 蛋白质结构预测小型开放式阅读框架 (smORFs)

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An Integrated Approach for Microprotein Identification and Sequence Analysis
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相关实验视频

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Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins

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科学领域:

  • 基因组学就是基因组学.
  • 蛋白质组学是指蛋白质组学.
  • 细菌生物学 细菌生物学

背景情况:

  • 由小型开放式读取框架 (smORF) 编码的微蛋白质占蛋白质"暗物质"的很大一部分.
  • 虽然功能性微蛋白在各个生命领域都已知,但细菌smORF研究不足.
  • 细菌中的基因间smORFs (ismORFs) 的特征特别差.

研究的目的:

  • 为了全面识别和表征肠杆菌家族的跨基因小开放阅读框架 (ismORFs).
  • 研究细菌ismORFs编码的微蛋白的潜在功能和相互作用.
  • 为未来的研究创建预测微蛋白家族的目录.

主要方法:

  • 对5,668个Enterobacteriaceae基因组进行全基因组分析,以识别ismORFs (15-70个编码子).
  • 计算分析以检测作用于ismORFs的净化选择.
  • 使用AlphaFold Multimer进行结构预测,以建模微蛋白相互作用.
  • 合成分析,结构预测和转录/翻译数据集成.

主要成果:

  • 在净化选择下识别了67,297个细菌ismORFs集群.
  • 编码的微蛋白质的特征主要是疏水性,潜在的跨膜性和最小结构性.
  • 预测微蛋白与邻近蛋白质的相互作用,表明调节作用,特别是在压力条件下.
  • 编制预测微蛋白家族的目录,并提供不同程度的支持性证据.

结论:

  • 细菌的ismORFs编码了一组多样化的微蛋白,具有潜在的调节功能.
  • 微蛋白可能在细胞过程中发挥关键作用,特别是在对环境压力的反应中.
  • 产生的目录为探索细菌微蛋白的生物学意义提供了宝贵的资源.