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运输温度对细胞活力和T细胞对细菌抗原反应的影响

Patpong Rongkard^1,2,3, Susanna J Dunachie^1,2,3, Barbara Kronsteiner^2,3

¹Faculty of Tropical Medicine, Mahidol-Oxford Tropical Medicine Research Unit, Mahidol University, Bangkok, 10400, Thailand.

Wellcome open research

|June 21, 2024

概括

将冷保存的外周血液单核细胞 (PBMC) 运送到 -196°C 保存细胞活力,并改善T细胞反应,相比 -80°C. 这一发现为临床试验中的样本管理提供了关键指导,特别是在资源有限的环境中.

科学领域:

免疫学免疫学免疫学
热带医学热带医学
临床研究临床研究

背景情况:

由T细胞分泌的干扰素-γ (IFN-γ) 对抗结核病和乳腺疹等病原体的免疫力至关重要.
在资源有限的热带地区进行临床研究需要可靠的方法来收集和运送生物样本.

研究的目的:

评估两个运输温度 (-80°C与196°C) 对外围血液单核细胞 (PBMC) 活力,组成和功能的影响.
为未来的临床研究提供关于最佳样本运输条件的指导.

主要方法:

来自梅里欧症患者的配对PBMC样品在-80°C (干冰) 或196°C (干货) 的温度下从泰国运往英国.
使用多色流细胞计分析了细胞活力和组成.
对*Burkholderia pseudomallei*的抗原特异IFN-γ反应通过IFN-γ酶相关免疫位点测定 (ELISpot) 进行测量.

主要成果:

与196°C相比,在-80°C的运输导致细胞活力略有下降,IFN-γ反应减少.
在运送在-80°C的样本中观察到单细胞和自然杀手细胞的减少.
在大多数样本中仍然可以检测到抗原特异性反应,即使是在-80°C下运输的样本中也是如此.

结论:

在 -196°C下冷保存的PBMCs的运输增强了细胞活力,恢复和T细胞对细菌抗原的反应.
虽然 -196°C是最优的,但从运送在 -80°C的样本中仍然可以获得有价值的数据.
这些发现对于临床试验中有效的样本管理至关重要,特别是在偏远或资源有限的环境中.

关键词:

伯克霍尔德里亚是假名的.埃利斯波特 (ELISpot) 是一个在线视频.IFN - 玛的使用情况这是PBMC的PBMC.冷保存的方法质性化症是什么运输运输运输运输是一个储存储存储存储存储存是一个

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