基于酸反应的分散型固体相提取法维皮拉维尔使用生物素从生物样本之前毛细管电泳分析的毛细管电泳分析
Elnaz Safari1, Behrouz Seyfinejad2,3, Mir Ali Farajzadeh4,5
1Pharmaceutical and Food Control Department, Faculty of Pharmacy, Tabriz University of Medical Sciences Tabriz Iran nematim@tbzmed.ac.ir mahnema@gmail.com.
RSC advances
|June 21, 2024
概括
一种新方法使用生物素作为绿色吸收剂从各种样本中提取法维皮拉维尔. 这种基于酸反应的技术提供了有效的样本准备分析.
科学领域:
- 分析化学 分析化学
- 环境化学环境化学
背景情况:
- 费维皮拉维尔的分析需要高效的提取方法.
- 绿色化学原理在分析过程中越来越重要.
研究的目的:
- 为favipiravir开发一种基于酸反应的新型分散固相提取 (d-SPE) 方法.
- 为了利用生物素作为一个环保的吸收剂,用于favipiravir提取.
主要方法:
- 开发了一种使用生物素作为吸附剂的d-SPE方法.
- 采用酸反应 (生物质子) 诱导吸附剂沉.
- 优化了提取参数,包括pH值,离子强度和超声波.
- 使用毛细体电泳二极管阵列检测 (CE-DAD) 确定法维皮拉维尔.
主要成果:
- 获得了法维皮拉维尔从250到3000 ng/mL (R2 = 0.9968) 的线性校准曲线.
- 证明了检测和量化能力的低极限.
- 表现出良好的重复性 (RSD ≤ 8.2%) 和可接受的提取回收.
- 成功地将该方法应用于的血和尿液样本.
结论:
- 开发的基于生物素的d-SPE方法是有效和高效的法维皮拉维尔提取.
- 该方法为复杂矩阵中favipiravir的确定提供了一个绿色和可行的替代方案.
- 该技术在常规分析和质量控制方面表现有前途.
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