来自尼泊尔的急性脑炎患者中检测到Gemykibivirus
Eans Tara Tuladhar1, Smita Shrestha1, Susan Vernon2
1Tribhuvan University Central Department of Biotechnology, Kathmandu, Nepal.
mSphere
|June 21, 2024
概括
在尼泊尔的急性脑炎综合征 (AES) 患者中发现了一种新型的gemykibivirus. 这一发现表明,gemykibivirus可能是脑炎的未被认可的原因,这促使进一步调查这种病毒病原体.
科学领域:
- 病毒学 病毒学
- 神经学 神经学
- 传染性疾病 传染性疾病
背景情况:
- 急性脑炎综合征 (AES) 是一个重大的全球卫生挑战,许多病例的病因不明.
- 日本脑炎病毒 (JEV) 占尼泊尔AES病例的一部分,但很大一部分仍然无法解释.
- 识别新型病毒病原体对于理解和管理脑炎爆发至关重要.
研究的目的:
- 确定尼泊尔AES的潜在病原体,特别是在未知病因的病例中.
- 从AES患者的脑脊液 (CSF) 中描述一种新发现的病毒.
- 为了确定这种病毒在AES患者更大的队列中的流行率.
主要方法:
- 甲基因组下一代测序 (mNGS) 用于分析来自AES患者的CSF样本.
- 开发了实时PCR测定,以检测CSF和血清样本中发现的病毒.
- 在阳性样本上进行了全基因组测序,以进一步表征病毒菌株.
主要成果:
- 在使用mNGS的8岁男性AES患者的CSF中发现了一种gemykibivirus.
- 完整的病毒基因组显示出与已知的gemykibiviruses具有高核酸相同性.
- 实时PCR证实了在12名其他AES患者 (11个CSF,1个血清) 中存在gemykibivirus DNA.
- 测序七个阳性样本提供了进一步的基因组数据.
结论:
- 这项研究确定了一种gemykibivirus作为尼泊尔脑炎的潜在病因因子.
- 这些发现表明,gemykibivirus可能是人类神经疾病的未被认可的原因.
- 需要进一步的研究来阐明AES. gemykibivirus的致病性和临床意义.
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