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通过一种新型的SPI-2注射体效应器调节沙门氏菌的毒性,该效应器与与dystrophin相关的蛋白质复合体相互作用

Xiu-Jun Yu¹, Haixia Xie², Yan Li³

¹Department of Infectious Disease, Centre for Bacterial Resistance Biology, Imperial College London, London, United Kingdom.

|June 21, 2024

概括

研究人员发现了一种新的沙门氏菌效应蛋白,SseM,它与宿主细胞结构相互作用. 这种蛋白质调节了小鼠的细菌生长和毒性,为沙门氏菌的病原性提供了洞察力.

科学领域:

微生物学微生物学
分子生物学分子生物学
病变的发生和发病.

背景情况:

沙门氏菌病原性岛屿2 (SPI-2) 注射体向宿主细胞输送效应物,以致病毒性.
了解沙门氏菌与宿主相互作用需要识别和描述SPI-2因子.

研究的目的:

通过蛋白质组方法识别新的SPI-2注射体效应体.
为了描述新发现的效应器的功能和宿主相互作用,SseM.

主要方法:

从临床Salmonella Enteritidis菌株中超分泌的SPI-2因子的蛋白质组分析.
同免疫沉和酵母双杂交试验以确定宿主蛋白相互作用.
在系统性疾病的小鼠模型中进行体内竞争测试.

主要成果:

发现了一种新的沙门氏菌效应因子,SseM,由SPI-2注射体转移.
通过PDZ结合基因,SseM与双氨酸相关蛋白质复合体 (DAPC) 的组成部分相互作用.
SseM调节沙门氏菌的毒性,抑制细胞内细菌的生长,并影响小鼠的全身感染.

结论:

SseM是一种新型的沙门氏菌效应剂,通过PDZ结合动机准宿主DAPC.
通过调节细菌生长和毒性,SseM在沙门氏菌病原发生过程中发挥作用.
这一发现扩大了已知的沙门氏菌效应因子以及它们与宿主相互作用的机制.

关键词:

在 DAPC 信号传输中.SPI-2注射了其中的一些.沙门氏菌是一种沙门氏菌.细菌效应器是一种细菌效应器.

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