葡萄球菌菌体内域操纵Endolysin,Endo88对Lytic效率和宿主范围的影响
Melvina Krishnan1, Hong Yun Tham1, Wan Ahmad Kamil Wan Nur Ismah1
1Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400, Serdang, Selangor, Malaysia.
Molecular biotechnology
|June 21, 2024
概括
菌体endolysin Endo88需要其所有域进行最大限度的抗菌活性,以对抗金黄色葡萄球菌和其他细菌. 移除域降低了光学效率,突出了每个组件对强效功能的重要性.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 菌体内素是对抗细菌病原体的有希望的抗微生物药物.
- 恩多88是一种向金黄色葡萄球菌的内溶酶,由CHAP,Amidase-2和SH3b域组成.
研究的目的:
- 为了研究细菌内个别域的功能作用,细菌endolysin Endo88.8.
- 确定域操纵如何影响Endo88的Lytic效率和主机范围.
主要方法:
- 构造的截断突变体 (CHAP,CHAPAmidase,CHAPSH3) 和模拟内素 (CHAPAmidase-Cpl7Cpl7,Endo88-Cpl7Cpl7).这些突变体中的一个是基因.
- 在大肠杆菌中表达和净化工程内素.
- 使用板溶解和度降低试验评估了活动和宿主范围.
主要成果:
- Endo88需要所有域进行最大限度的光学活动.
- 截断的突变体显示效率降低,但保留了原始宿主范围.
- 氨基酶-2和SH3b域表现出最佳功能的相互依赖性.
- 化学构造物表现出功能降低和对Streptococcus agalactiae的活性丧失.
结论:
- 所有Endo88的域都对其最大的抗微生物疗效至关重要.
- 域间的相互依赖对于最大限度地提高内解素的功能至关重要.
- 获得的见解可以指导工程内素作为抗微生物药物对抗多药耐药细菌的开发.
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