在2型糖尿病患者中,PAR水平介导了ROS和炎症反应之间的联系
Michele Zampieri1, Katsiaryna Karpach1, Gerardo Salerno2
1Department of Experimental Medicine, Faculty of Medicine and Dentistry, Sapienza University of Rome, 00161, Rome, Italy.
Redox biology
|June 21, 2024
概括
过度激活多ADP-ribosyl化 (PARylation) 与2型糖尿病 (T2DM) 中的低血糖控制和氧化应激有关. 增加的PAR水平与T2DM患者的炎症标志物相关.
科学领域:
- 生物化学 生化学
- 免疫学 免疫学 免疫学
- 内分泌学 在内分泌学.
背景情况:
- 2型糖尿病 (T2DM) 涉及破坏葡萄糖平衡,氧化应激和炎症.
- 聚ADP-ribosyl) 化 (PARylation) 是一种在T2DM病理生理学中涉及的翻译后修饰.
- 在人类T2DM中,链接PARylation,氧化应激和炎症的临床证据有限.
研究的目的:
- 研究T2DM患者中氧化应激,PARylation和炎症反应之间的关系.
- 探索PARylation在T2DM病理生理学中的临床相关性.
主要方法:
- 在61名T2DM患者和48名对照组中进行了横截面研究.
- 使用ELISA对外围血液单核细胞 (PBMC) 中的PAR水平的量化.
- 评估血和PBMC中的氧化应激标志物 (d-ROM,抗氧化能力).
- 在PBMC中通过qPCR测量抗氧化酶 (SOD1,GPX1,CAT) 和炎症媒介 (IL6,TNF-α,CD68,MCP1) 基因表达.
主要成果:
- T2DM患者在PBMC中表现出升高的PAR水平和增加的血d-ROMs.
- PAR水平和d-ROM之间的积极关联,表明氧化应激和PAR代谢之间的联系.
- 氧化应激 (d-ROMs) 调解了HbA1c和PAR水平之间的关联.
- 升高的PAR水平与PBMC中益炎性细胞因子IL6和TNF-α的表达增加相关.
结论:
- 过度激活的PARylation与T2DM中血糖控制不良和氧化应激有关.
- 增加的PAR水平与T2DM上调的炎症介质相关.
- 需要进一步的研究来验证发现并探索T2DM中PARylation的临床影响.
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