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快速响应和高度选择性的NIR光探针用于检测食品样本和活细胞中的硫化

Xuejing Xu¹, Yuling Zeng¹, Haichang Ding¹

¹Jiangxi Key Laboratory of Organic Chemistry, Jiangxi Science and Technology Normal University, Nanchang 330013, PR China.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy

|June 21, 2024

概括

开发了一种新的近红外探测器WX-HS,用于检测硫化 (H2S). 这种探测器可以快速,灵敏地检测H2S,这对于监测食品新鲜度和细胞成像至关重要.

科学领域:

化学传感器是一种化学传感器.
生物医学诊断生物医学诊断
食品科学食品科学食品科学

背景情况:

硫化 (H2S) 是生物系统中一个关键的信号分子.
H2S水平与富含蛋白质的食物的新鲜度相关.
现有的H2S检测方法可能缺乏灵敏度或速度.

研究的目的:

设计和合成一种新的近红外 (NIR) 光探测器用于H2S检测.
在灵敏度,反应时间和特异性方面评估探头的性能.
评估探头在食品新鲜度监测和生物成像中的适用性.

主要方法:

开发一种基于氨酸衍生物的NIR探针 (WX-HS),其中包含2,4-丁二硫酸盐 (DNBS) 反应组.
用光谱分析来描述探测器对H2S的光反应.
在食品氧化模型和A549细胞中进行H2S检测的体外试验.

主要成果:

探测器WX-HS表现出一个快速 (90秒) 和敏感的光启动响应H2S在660nm.
在H2S相互作用时观察到一种明显的颜色变化,从非光到色红色.
WX-HS成功检测到食物氧化过程中产生的H2S,并使低细胞毒性A549细胞中的H2S成像成为可能.

结论:

探测器WX-HS是一个高度有效的工具,用于敏感和快速的H2S检测.
它监测食物腐烂和执行细胞成像的能力突出了它的多功能性.
WX-HS为H2S相关的诊断和研究提供了一个有希望的平台.

关键词:

细胞成像细胞成像食品样本食品样本 H (((2) S S 的情况.NIR光探测器进行光探测.芬诺提亚 (Phenothiazine) 是一种药物.

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