影响结核病患者唾液中Mycobacterium结核菌脂体含量的变异性的宿主和病原体因素:一项观察性研究
Baye G Tarekegn1, Leopold D Tientcheu2, Jonathan Decker3
1Department of Respiratory Sciences, University of Leicester, Leicester, UK; Department of Medical Microbiology, University of Gondar, Gondar, Ethiopia.
The Lancet. Microbe
|June 21, 2024
概括
唾液中的Mycobacterium结核菌脂体 (LB+) 含量与治疗结果有关. 主体产生的氧化 (NO) 和细菌菌株影响LB+水平,表明LB+是结核病治疗反应的预测生物标志物.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 临床医学 临床医学
背景情况:
- 唾液中Mycobacterium结核病细胞与脂质体的高比例与治疗失败相关.
- 影响脂体阳性 (%LB+) M.结核病频率变化的因素尚不清楚.
- 宿主产生的氧化 (NO) 和M.结核菌株被假定会影响液%LB+.
研究的目的:
- 为了研究宿主产生的NO,M.结核菌株和唾液%LB+频率之间的关联.
- 为了确定唾液%LB+是否可以作为结核病治疗反应的预测生物标志物.
主要方法:
- 在埃塞俄比亚和冈比亚进行的观察性研究,通过显微镜测量液%LB+.
- 在埃塞俄比亚,作为宿主NO生物标志物,评估了部分呼出的氧化 (FeNO).
- 使用spoligotyping和体外NO暴露实验确定了M.结核病菌株的差异.
主要成果:
- 在埃塞俄比亚患者中,唾液%LB+与log10 FeNO (r2=0.28;p<0.0001) 相相关.
- 结核菌株在体外对NO的反应有所差异,影响了体内的脂质含量和tgs1mRNA.
- 在治疗前,更高的唾液%LB+频率与治疗反应较差有关.
结论:
- 结核菌株和宿主产生的NO暴露与唾液%LB+有关.
- 由细菌菌株影响的液%LB+频率显示出作为结核病治疗反应预测生物标志物的潜力.
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