在海洋浮游生物物质连续体中, prokaryotic 活动的模式
Encarna Borrull1, Mireia Mestre1, Cèlia Marrasé1
1Department of Marine Biology and Oceanography, Institut de Ciències del Mar, CSIC. Pg Marítim de la Barceloneta 37-49, E08003 Barcelona, Catalunya, Spain.
The Science of the total environment
|June 21, 2024
概括
这项研究揭示了 prokaryotic 活动在较小的大小分数中是最高的,但每个细胞的特定酶活性随着粒子大小的增加而增加. 这强调了考虑所有尺寸分数对于准确的微生物评估的重要性.
科学领域:
- 海洋微生物生态学
- 生物地质化学生物地质化学
- 海洋学 海洋学 海洋学
背景情况:
- Prokaryotic 的丰富性和活动通常分为自由生活和粒子附着的部分.
- 有机物质存在于一个连续的大小中,影响异质性 prokaryote 的生长.
- 准确评估 prokaryotic 社区活动需要考虑所有环境大小分数.
研究的目的:
- 在各种尺寸分数中调查 prokaryotic 丰富性,异质性活动和细胞外酶活动.
- 为了确定微生物活动和酶特异性如何随着沿海海洋环境中的颗粒大小而变化.
主要方法:
- 从西北地中海的一个沿海地点采集的样本.
- 测量 prokaryotic 丰富度 (PA) 和白蛋白的结合.
- 在尺寸分片样本 (孔径大小为0.2-0.8-3-5-10μm) 中分析细胞外酶活性.
主要成果:
- 微小于0.8μm的部分含有大部分细胞 (91.6%) 和氨酸 (72.2%).
- 溶解的分量 (<0.2μm) 显示出最高的细胞外总酶活性.
- 每个细胞的特定酶活性在颗粒 (>0.8微米) 中比自由活分数 (0.2-0.8微米) 高100-1000倍.
结论:
- 5-10μm的分量表现出氨酸合并和大多数酶的最高特异性活性.
- 超过10μm的分量显示出更高的特定基托巴酶活性,这表明动物浮游生物衍生的有机物质水解的专业化.
- 考虑到所有尺寸分数,可以更准确地表示海洋生态系统中 prokaryotic 社区结构和功能.
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