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相关概念视频

Sample Preparation for Analysis: Overview01:21

Sample Preparation for Analysis: Overview

Sample preparation is an essential step in the analytical process. It involves preparing a sample so that it can be analyzed accurately. The goal is to extract the analyte, the substance you want to measure, from the sample while removing any components that may interfere with the analysis. Sample preparation techniques vary depending on the physical state of the sample.
Bulk or large solid samples are typically reduced in size using grinding, crushing, or milling techniques to increase the...

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相关实验视频

Updated: Jun 26, 2026

Cell-Lineage Guided Mass Spectrometry Proteomics in the Developing Frog Embryo
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单细胞蛋白质组学无标签样本制备方法

David Hartlmayr1, Claudia Ctortecka2, Rupert Mayer3

  • 1Cellenion, Lyon, France.

Methods in molecular biology (Clifton, N.J.)
|June 21, 2024
PubMed
概括
此摘要是机器生成的。

单细胞蛋白质组学 (SCP) 方法被优化,以改善回收和工作流的稳定性. 本协议使用cellenONE平台,在LC-MS分析前进行高效,无标签的样本准备.

关键词:
自动化 自动化 自动化没有标签的无标签.单细胞蛋白质组学 单细胞蛋白质组学在微米升以下的样本准备过程中凯伦ONE® 公司 凯伦ONE®蛋白质CHIP LF 48 48 这是一个很好的方法.

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科学领域:

  • 蛋白质组学是指蛋白质组学.
  • 细胞生物学 细胞生物学
  • 生物化学 生物化学

背景情况:

  • 单细胞蛋白组学 (SCP) 对于理解细胞异质性至关重要.
  • 每个细胞的蛋白质数量有限,需要高效的样本准备.
  • 现有的SCP方法面临的挑战是损失和放大限制.

研究的目的:

  • 为无标签的单细胞蛋白质组学样本制备提供一个优化的协议.
  • 增强回收,提高SCP工作流程的稳定性.
  • 详细说明cellenONE平台和proteoCHIP LF 48基板的使用情况.

主要方法:

  • 利用cellenONE平台进行自动化,低量样本处理.
  • 为了高效的捕获,使用了proteoCHIP LF 48基质.
  • 实施了无标签样本准备策略.
  • 与高性能液体染色学质谱学 (HPLC-MS) 结合样本制备.

主要成果:

  • 通过减少加工量和尽量减少手工转移,显著改善回收.
  • 提高了单细胞蛋白质组学工作流的整体稳定性.
  • 证明了无标签SCP分析的可行协议.

结论:

  • 描述的协议为无标签的单细胞蛋白质组学提供了一个强大而有效的方法.
  • 优化样本准备对于克服SCP中低蛋白质输入的挑战至关重要.
  • 这种方法可以通过单细胞蛋白质组学更深入地了解细胞异质性.