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Updated: Jun 23, 2025

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
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EquiCP:通过质谱测量以同位素标记多重复合为目标的单细胞蛋白质组学

Prajakta Naval1, Laura Pérez Pañeda1, Maria Athanasopoulou1

  • 1Division of Chemistry I, Department of Medical Biochemistry & Biophysics, Karolinska Institutet, Stockholm, Sweden.

Methods in molecular biology (Clifton, N.J.)
|June 21, 2024
PubMed
概括

这项研究引入了针对单个人类细胞的向蛋白质组学方法. 用合成替换载体蛋白质增强了质谱分析中的蛋白质识别和量化.

关键词:
质谱测量质量谱测量样品的准备 样品的准备单细胞蛋白质组学 单细胞蛋白质组学合成类的合成.坦德姆群体标签 坦德姆群体标签有针对性的蛋白质组学.

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Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
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Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
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