合成气和热解水性凝聚物的两阶段转化为L-酸盐
Alberto Robazza1, Flávio C F Baleeiro2, Sabine Kleinsteuber2
1Institute of Process Engineering in Life Sciences 2: Electro Biotechnology, Karlsruhe Institute of Technology - KIT, 76131, Karlsruhe, Germany.
Biotechnology for biofuels and bioproducts
|June 21, 2024
概括
混合工艺从有机废物中回收超过50%的能量,转化为短链碳酸盐. 这项研究表明,使用混合微生物培养物同时进行合成气封存,废物排毒和有价值的化学品生产.
科学领域:
- 生物技术和生物工程 生物技术和生物工程
- 环境科学 环境科学
- 微生物学 微生物学
背景情况:
- 混合热化学-生物过程为从有机废物中提升碳和能量回收提供了潜力.
- 热解水凝析物 (PAC) 由于其毒性而带来挑战,阻碍了有效的资源回收.
研究的目的:
- 评估多功能工艺的碳和能量回收潜力,以同时进行合成气封存和PAC排毒.
- 用于从合成气和PAC共发酵中生产短链碳酸盐.
- 评估混合培养共同发酵的美索菲尔和热友条件.
主要方法:
- 在同样的反应器中,在中性 (37°C) 和热性 (55°C) 条件下,与越来越高的PAC加载率一起,合成气和PAC的共同发酵.
- 16S rRNA基因扩增序列测序用于分析微生物社区结构.
- 一个使用Aspergillus oryzae的第二阶段反应器,用于将碳酸盐转化为L-酸盐.
主要成果:
- 在中性和热性过程中,至少50%的能量从合成气和PAC中回收到短链碳酸盐中.
- 介质共发酵抑制了甲基生成,产生了酸盐,乙醇和丁酸盐,其中Clostridium sensu stricto 12占主导地位 (>90%).
- 热友性过程涉及各种微生物进行芳香物降解和甲基生成;高生物质对于高PAC负载的稳定性至关重要.
- Aspergillus oryzae成功地将碳酸盐转化为L-酸盐,证实了PAC排毒低于抑制水平,其产量为0.26 ± 2.2mol L-酸盐/mol碳酸盐.
结论:
- 带有无氧混合培养的多功能反应器可以同时固定碳,排毒废水,并产生碳酸盐中间体.
- 以碳酸盐的形式回收的能量可以在随后的发酵阶段被利用.
- 这种方法表明有机废物流的有效资源回收和废物利用.
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