将pH控制集成到Chi.Bio反应器中,并通过小规模的酶性聚乙烯甲酸盐进行水解
Mackenzie C R Denton1,2, Natasha P Murphy1,2, Brenna Norton-Baker1,2
1Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory, Golden, Colorado 80401, United States.
Biochemistry
|June 22, 2024
概括
可负担得起的小型生物反应器现在提供连续的pH控制. 这种增强可以实现高效的生化反应和培养,显著减少酶的使用.
科学领域:
- 生物技术是生物技术.
- 化学工程是化学工程的重要组成部分.
- 合成生物学 合成生物学
背景情况:
- 可负担得起和易于使用的小型生物反应器对于研究至关重要.
- 开源自动化生物反应器Chi.Bio系统以较低的成本商用.
- 之前的Chi.Bio版本提供了光学监测,和温度控制,可达到30毫升尺度.
研究的目的:
- 通过整合连续的pH监测和控制来扩大Chi.Bio系统的能力.
- 为了证明pH控制的生物反应在小尺度上的实用性.
主要方法:
- 硬件修改包括集成低成本商用pH电路和调整Chi.Bio头板.
- 软件修改包括在用户界面中实现闭环pH反控制和pH控制模块.
- 通过使用割酶酶去聚合聚乙烯二甲酸盐 (PET) 证明有用性,将小规模Chi.Bio结果与250毫升生物反应器反应进行比较.
主要成果:
- 连续的pH监测和控制成功地集成到Chi.Bio系统中.
- 在Chi.Bio中小规模的PET脱聚合显示出与250毫升生物反应器反应相比,PET转化和速率在统计上相当.
- Chi.Bio系统实现了对PET水解的净化酶需求的20倍减少.
结论:
- 廉价的修改使Chi.Bio反应器的pH值可以控制,扩大了它们用于生物化学反应和生物栽培的应用范围.
- 改进的Chi.Bio系统为小规模生物反应提供了一个具有成本效益的平台,并具有精确的pH管理.
- 为Chi.Bio开发的pH控制模块可以适应其他生物反应器平台.
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