使用无损离子操纵结构来阐明尼塔类比原体的气相行为 离子移动性-轨道式质谱仪
Adam L Hollerbach1, Vivian S Lin1, Yehia M Ibrahim1
1Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington 99354, United States.
Journal of the American Society for Mass Spectrometry
|June 22, 2024
概括
新型合成阿片类药物称为尼塔是强效的,在过量服用的情况下报告不足. 离子移动性谱法 (IMS) 提供了一种有前途的方法,用于在毒理学中快速,无参考的识别.
科学领域:
- 法医毒理学 法医毒理学
- 分析化学 分析化学
- 质谱测量质量谱测量
背景情况:
- 尼塔是强大的新型合成阿片类药物 (NSO),在药物过量病例中越来越多地发现.
- 它们的高强度和缺乏常规检测导致报告不足.
- 目前的识别方法通常需要参考标准,延迟分析.
研究的目的:
- 介绍第一个离子移动性光谱 (IMS) 和碰撞截面 (CCS) 测量十四种尼塔类型的测量结果.
- 探索IMS在毒理学中无基准识别尼塔的潜力.
- 用IMS,溶剂和碎片化研究来研究尼塔的气相行为.
主要方法:
- 用于无损离子操纵 (SLIM) - 轨道轨道质谱仪用于IMS和CCS测量的结构.
- 研究了电喷离子化 (ESI) 溶液中的水对IMS分布的影响.
- 进行IMS-MS/MS实验,分析碎片化模式和气相原体.
- 采用循环IMS来解决结构上相似的尼塔异构体.
主要成果:
- 所有14种尼塔都显示出两种不同的IMS分布.
- 将水添加到ESI溶液中改变了IMS分布强度,特别是对于缺乏基的尼塔.
- IMS-MS/MS显示了高和低流动性分布之间的碎片化模式的差异,这表明有三个气相原体.
- 使用循环IMS实现了尼塔异构体的部分分离.
结论:
- IMS提供了一种强大的工具,可以快速,无参考地识别新兴的尼塔和其他NSO.
- 通过IMS了解尼塔气相行为,有助于其结构的阐明.
- 与MS工作流程集成的高分辨率IMS分离可以显著提高NSO在法医实验室的检测率.
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