相关实验视频

Updated: Jun 23, 2025

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome

Published on: June 15, 2016

在亚临床甲状腺功能低下症相关流产中的JPT2作为转录辅助因子:LEPR/STAT3激活的参与

Y-Y Zhou¹, S-Y Zhao¹, F-J Huang¹

¹Department of Endocrinology and Metabolism, the First Affiliated Hospital of Zhengzhou University, No. 1, East Jianshe Road, Zhengzhou, 450000, Henan Province, People's Republic of China.

Journal of endocrinological investigation

|June 22, 2024

概括

在亚临床甲状腺功能低下症 (SCH) 中,木星微管相关同源2 (JPT2) 的下调. 恢复JPT2有助于热囊细胞功能,这表明JPT2缺乏有助于SCH相关的怀孕流产.

科学领域:

生殖生物学生殖生物学
内分泌学在内分泌学.
基因组学就是基因组学.

背景情况:

亚临床甲状腺功能低下症 (SCH) 与植入失败和 trofhoblast 功能障碍有关.
从SCH大鼠中流产的胎盘的转录组分析揭示了下调的木星微管相关同类2 (JPT2).

研究的目的:

调查木星微管相关同类2 (JPT2) 在亚临床甲状腺功能低下症 (SCH) 相关流产中的作用.
探索JPT2对热囊细胞的功能影响.

主要方法:

通过甲状腺切除术和levothyroxine管理诱导的亚临床甲状腺功能低下症 (SCH) 鼠标模型.
流产胎盘的转录基因分析.
在HTR-8/SVneo热囊细胞上进行功能增益和丧失实验,以评估JPT2的影响.

主要成果:

在SCH大鼠中流产的胎盘中,JPT2显著下调.
过度表达JPT2增强了 trofhoblast 细胞的增殖,迁移,入侵,球形形成和附着.
抑制JPT2抑制了这些 trofhoblast细胞的功能.
JPT2加速了瘦素受体 (LEPR) 转录,并激活了STAT3信号通路.

结论:

与SCH相关的流产中低调的JPT2有助于 trofhoblast细胞功能障碍.
通过LEPR/STAT3轴,JPT2促进 trofhoblast细胞的增殖,迁移,入侵和附着.
JPT2作为转录辅助因子,其低表达可能会导致SCH患者的流产.

关键词:

这是入侵.木星微管相关的同类物2 木星微管相关同类物2 勒普丁受体的受体是什么?在STAT3中,我们可以使用STAT3.热细胞细胞的细胞.

更多相关视频

Generation of a Mouse Spontaneous Autoimmune Thyroiditis Model

Generation of a Mouse Spontaneous Autoimmune Thyroiditis Model

Published on: March 17, 2023

Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts

Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts

Published on: October 9, 2016

相关概念视频

Synthesis and Regulation of Thyroid Hormones

Synthesis and Regulation of Thyroid Hormones

Low blood levels of the thyroid hormones — triiodothyronine (T3) and thyroxine (T4) — signal the hypothalamus to release the thyrotropin-releasing hormone (TRH). TRH then reaches the pituitary gland and stimulates the release of thyroid-stimulating hormone(TSH) into the bloodstream.
Upon reaching the thyroid gland, TSH stimulates the follicular cells' active uptake of iodide ions from the blood. The ions diffuse to the apical surface of the cells and are oxidized to iodine. The...

General Transcription Factors

General Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...

The JAK-STAT Signaling Pathway

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...

Master Transcription Regulators

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...

Non-LTR Retrotransposons

Non-LTR Retrotransposons

As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...

TGF - β Signaling Pathway

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...