Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Real Time RT-PCR02:57

Real Time RT-PCR

57.2K
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
57.2K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

mRNA Delivery by Lipoamino Fatty Acid-Peptide Polyplexes in Different Lung Cell Models and Lungs.

Polymers·2026
Same author

Protocol for co-culture of murine bone marrow-derived dendritic cells with OT-I mouse CD8<sup>+</sup> T cells to evaluate immune activation efficacy.

STAR protocols·2026
Same author

Meta-Learning as a Promising Strategy for Lipid Nanoparticle Optimization and Ionizable Lipid Discovery.

Nano letters·2026
Same author

Lipid@polymer hybrid nanoparticles for efficient siRNA transport across the lung barriers: Mechanistic insights into the role of Ionizable lipids.

Journal of colloid and interface science·2026
Same author

New insights into the intrinsic tryptophan fluorescence emission spectra of IgG antibodies and the impact of protein unfolding and aggregation.

European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V·2026
Same author

A hybrid polymeric system for pulmonary mRNA delivery: Advancing mucosal vaccine development.

Cell biomaterials·2026

相关实验视频

Updated: Jun 23, 2025

Detection of Intracellular Gene Expression in Live Cells of Murine, Human and Porcine Origin Using Fluorescence-labeled Nanoparticles
08:14

Detection of Intracellular Gene Expression in Live Cells of Murine, Human and Porcine Origin Using Fluorescence-labeled Nanoparticles

Published on: November 13, 2015

11.1K

在纳米粒子中检测RNA的光技术.

Simone P Carneiro1,2, Joschka T Müller1, Olivia M Merkel3,4,5

  • 1Department of Pharmacy, Pharmaceutical Technology and Biopharmacy, Ludwig-Maximilians-University Munich, Munich, Germany.

Methods in molecular biology (Clifton, N.J.)
|June 22, 2024
PubMed
概括

在纳米药物中精确量化核酸至关重要. 本研究详细介绍了使用RiboGreen和SYBR Gold的协议,用于评估脂质纳米颗粒和多复合体/微粒复合体的封装效率.

关键词:
封装效率 封装效率是指封装效率是指封装效率.里博格林 (RiboGreen) 是一个绿色的城市.这就是SYBR黄金.如果RNA-脂质纳米颗粒.如果RNA-micelleplexes复杂.的 siRNA 多重复合体.

更多相关视频

Fluorescent End-Labeling and Encapsulation of Long RNAs for Single-Molecule FRET-TIRF Microscopy
10:59

Fluorescent End-Labeling and Encapsulation of Long RNAs for Single-Molecule FRET-TIRF Microscopy

Published on: October 18, 2024

618
Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
12:20

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Published on: August 6, 2014

11.8K

相关实验视频

Last Updated: Jun 23, 2025

Detection of Intracellular Gene Expression in Live Cells of Murine, Human and Porcine Origin Using Fluorescence-labeled Nanoparticles
08:14

Detection of Intracellular Gene Expression in Live Cells of Murine, Human and Porcine Origin Using Fluorescence-labeled Nanoparticles

Published on: November 13, 2015

11.1K
Fluorescent End-Labeling and Encapsulation of Long RNAs for Single-Molecule FRET-TIRF Microscopy
10:59

Fluorescent End-Labeling and Encapsulation of Long RNAs for Single-Molecule FRET-TIRF Microscopy

Published on: October 18, 2024

618
Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
12:20

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Published on: August 6, 2014

11.8K

科学领域:

  • 生物医学工程 生物医学工程
  • 纳米技术 纳米技术
  • 分子生物学分子生物学

背景情况:

  • 核酸输送系统,如脂质纳米颗粒和多复合体/微复合体,对于治疗疾病至关重要.
  • 核酸货物的精确量化对于纳米医学的安全性和有效性至关重要.
  • 在这些系统中,RiboGreen和SYBR Gold是RNA量化标准方法.

研究的目的:

  • 为评估纳米载体中核酸的封装效率提出一个全面的协议.
  • 提供纳米粒子制备和表征的详细说明.
  • 为了比较RiboGreen和SYBR Gold对量化未封装小干扰RNA (siRNA) 的灵敏度.

主要方法:

  • 使用RiboGreen测定脂质纳米颗粒的方法.
  • 使用SYBR金色测定多复合体/微复合体.
  • 包括纳米粒子制备,表征和定量封装和未封装siRNA的协议.

主要成果:

  • 建立了准确的封装效率确定协议.
  • 证明了RiboGreen和SYBR Gold对siRNA量化的比较敏感性分析.
  • 提供了核酸纳米医药开发中质量控制的框架.

结论:

  • 提出的协议允许精确评估核酸封装效率.
  • 对比分析有助于根据纳米载体类型和灵敏度要求选择最佳量化方法.
  • 标准化方法对于推动开发安全有效的基于核酸的纳米疗法至关重要.