基于脂质的纳米粒子用于RNA输送
Xiomara Calderón-Colón1, Richard Egan2
1Asymmetric Operations Sector, Applied Biological Sciences, The Johns Hopkins University Applied Physics Laboratory, Laurel, MD, USA. Xiomara.Calderon-Colon@jhuapl.edu.
Methods in molecular biology (Clifton, N.J.)
|June 22, 2024
概括
本章详细介绍了一种简单的批量混合方法,用于在脂质纳米颗粒中封装核糖核酸 (RNA). 它还涵盖了评估RNA封装效率和用于输送应用的纳米粒子特性的基本表征技术.
科学领域:
- 纳米技术 纳米技术
- 生物医学工程 生物医学工程
- 药物输送系统 药物输送系统
背景情况:
- 基于纳米粒子的系统对于保护和传递活性分子至关重要.
- 脂质纳米颗粒 (LNP) 是体外和体内应用的重要选择.
- 核糖核酸 (RNA) 的有效输送仍然是治疗学中的一个关键挑战.
研究的目的:
- 描述一种简单的批量混合方法,用于在脂质纳米颗粒中封装RNA.
- 介绍用于评估RNA封装效率的表征技术.
- 概述评估产生的纳米粒子物理化学性质的方法.
主要方法:
- 实施一种用于RNA封装的简单批量混合技术.
- 使用特定的测试来确定封装效率.
- 应用物理化学表征方法用于纳米粒子分析.
主要成果:
- 使用所述的批量混合方法成功封装RNA.
- 通过应用技术实现的封装效率的量化.
- 用RNA载脂纳米粒子的关键物理化学性质的表征.
结论:
- 本次介绍的批量混合方法提供了一个简单的方法,用于在脂质纳米颗粒中封装RNA.
- 描述技术对于验证这些传递系统的质量和性能至关重要.
- 这种方法和表征方法支持开发基于RNA的治疗方法.
相关概念视频
02:15Experimental RNAi
6.1K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.1K
01:23RNA Interference
26.0K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
26.0K
02:30siRNA - Small Interfering RNAs
16.7K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
16.7K