对于 Lactococcus paracarnosus 的不同气体成分和与 Brochothrix thermosphactacta 的共同培养的转录组反应
Victoria Werum1, Matthias Ehrmann1
1Lehrstuhl für Mikrobiologie, Technische Universität München, Gregor-Mendel-Straße 4, 85354 Freising, Germany.
International journal of food microbiology
|June 22, 2024
概括
乳球球菌的新陈代谢和腐烂潜力受到包装气体混合物的显著影响. 高氧含量可能会导致异味,而特定的气体成分会抑制竞争性细菌,如Brochothrix thermosphacta.
科学领域:
- 食品微生物学 食品微生物学
- 细菌的新陈代谢
- 肉类科学 肉科学
背景情况:
- 乳球球菌 (Lactococcus paracarnosus) (Lc. 葡萄球菌) 是一种可怕的菌种. 帕拉卡诺索斯) 和Lc. 肉类微生物在肉类微生物群中很常见.
- 它们在肉类腐烂中的作用是有争议的;一些菌株抑制腐烂细菌,而另一些菌株则导致腐烂.
- 在 Lc. 帕拉卡诺苏斯 DSM 111017 之所以被选中,是因为它能够抑制肉类破坏者,如 Brochothrix thermosphacta.
研究的目的:
- 研究通过Lc.生物保护的机制. 帕拉卡诺索斯. 帕拉卡诺索斯.
- 确定如何Lc. 帕拉卡诺索斯有助于肉类腐烂.
- 分析不同气体混合物和B. thermosphacta对Lc.的影响. 帕拉卡诺索斯生长和基因表达.
主要方法:
- 培养Lc. 的文化. paracarnosus DSM 111017 在4°C的肉模拟agar上.
- 将培养物暴露在四种不同的头部气体混合物中:模拟空气,HiOx-MAP,nonOx-MAP和模拟真空.
- 评估Brochothrix thermosphacta TMW 2.2101存在的影响.
- 分析Lc.中的基因表达变化. 帕拉卡诺索斯. 帕拉卡诺索斯.
主要成果:
- 气体混合物显著改变了Lc. 帕拉卡诺索斯基因表达,影响代谢途径和破坏代谢物生产.
- 在高氧气下,Lc. 帕拉卡诺索斯偏好产生的乙/二甲酸比乳酸,可能导致黄油淡味.
- 特定气体混合物 (非Ox-MAP,模拟真空) 和Lc. 帕卡诺索斯抑制了B. thermosphacta的生长,但B. thermosphacta没有影响Lc. 帕拉卡诺索斯基因调节.
结论:
- 包装气体的成分对Lc产生重大影响. 的代谢,腐烂的贡献,以及其抑制B. thermosphacta的能力.
- 这种菌株可能不适合用于高氧包装,因为可能会产生异味.
- 需要进一步的研究,以充分阐明抑制和破坏的机制.
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