通过一种新的ISCR元素,ISCR28捕获armA
Min Yuan1, Lu Nie2, Zhenzhou Huang1
1National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
International journal of antimicrobial agents
|June 22, 2024
概括
插入序列ISCR28表现出转移酶活性,并集成到格兰负生物体中. 它的特定DNA插入偏好和与抗性基因共同转移的潜力突出显示了它的生物学意义.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 微生物学 微生物学
背景情况:
- ISCR28属于类似IS91的插入序列家族.
- 插入序列是移动的遗传元素,在基因组进化和基因转移中起着重要作用.
研究的目的:
- 描述ISCR28.8的功能活动和遗传背景.
- 阐明ISCR28插入和调动的机制.
主要方法:
- 来自GenBank的60个含有ISCR28的序列的生物信息分析.
- 识别目标DNA序列和遗传背景.
- 使用等离子体对转移酶活性进行实验验证.
主要成果:
- ISCR28是1,708 bp,其中1,293 bp是转体酶编码的开放读取框架.
- 在各种格拉姆阴性生物中发现了自然存在的ISCR28插入.
- 在terIS端观察到对5'-GXXT-3'的插入偏好.
- 有证据表明ISCR28可以与抗生素耐药性基因共同转移.
结论:
- ISCR28是一种具有特定插入位偏好的功能转移酶.
- 基因组上下文和序列改变影响ISCR28调动和共同转移.
- ISCR28的活性有助于水平基因转移和基因组可塑性.
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