高分辨率血清型鉴定揭示了土耳其工业沙门氏菌监测方面的挑战
Emily E Cason1, Anna V Carlson2, Angela L Siemens2
1Poultry Diagnostic and Research Center, University of Georgia, 953 College Station Rd, Athens, GA 30602, USA.
Journal of food protection
|June 22, 2024
概括
土耳其的沙门氏菌污染是一个持续的问题,检测量从养殖场到加工场有很大差异. 在火产品中追踪特定的血清病毒为有效的食品安全监督带来了复杂的挑战.
科学领域:
- 食品安全 食品安全
- 微生物学 微生物学
- 兽医公共卫生 兽医公共卫生
背景情况:
- 沙门氏菌仍然是食源性疾病的重要原因,火产品与人类病例的显著比例有关.
- 在家禽加工业现有的沙门氏菌控制措施尚未完全消除火产品中的污染.
研究的目的:
- 分析美国农业部食品安全和检查局 (USDA-FSIS) 关于火沙门氏菌验证的数据.
- 在整个火群的生产和加工过程中调查沙门氏菌的流行率和血清细胞的多样性.
主要方法:
- 对美国农业部-FSIS土沙门氏菌流行数据 (2016-2022) 的分析.
- 从22个火群中采集和测试样本,包括农场的靴,加工厂样本 (翼尖,机械分离的火,土),使用qPCR和培养.
- 使用CRISPR-SeroSeq技术进行血清细胞鉴定和表征.
主要成果:
- 美国农业部-FSIS数据显示,从2016年到2022年,土中的沙门氏菌患病率平均为15.9%,主导血清病毒频繁变化.
- 在qPCR和培养结果之间观察到显著差异 (33.2%的PCR阳性,培养负).
- 在农场的8/22个群体中检测到沙门氏菌,但在加工阶段的21/22个群体中检测到沙门氏菌,最终产品中有18/22个阳性. 在预热阶段 (53.8%) 患病率最高.
- 通过CRISPR-SeroSeq识别了18种血清病毒,其中35.1%的样本含有多种血清病毒. 在16个群体中,没有发现上游的最终产品血清.
- 最终产品中的Serovar Typhimurium通常是活衰减的疫苗菌株. 在农场和冷前的沙门氏菌并不总是预测最终产品的污染.
结论:
- 土耳其的沙门氏菌污染和血清菌谱是复杂和动态的,挑战了传统的追踪方法.
- 分子和培养方法之间的差异会影响监测的准确性.
- 目前的采样点可能无法有效地代表最终火产品中存在的沙门氏菌血清型,这表明需要改进监测策略.
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