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相关概念视频

The Replisome03:01

The Replisome

33.4K
DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
33.4K
Translesion DNA Polymerases02:10

Translesion DNA Polymerases

9.9K
Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
TLS polymerases are found in all three domains of life - archaea, bacteria, and eukaryotes. Of the different classes of TLS polymerases, members of the Y family are fitted with specialized structures that...
9.9K
Proofreading01:31

Proofreading

6.2K
Synthesis of new DNA molecules is carried out by the enzyme DNA polymerase, which adds nucleotides on the daughter strand complementary to the template DNA strand. DNA polymerase has a higher affinity to add the correct base and ensures fidelity during DNA replication. Furthermore,  it exhibits proofreading activity during replication, using an exonuclease domain that cuts off incorrect nucleotides from the nascent DNA strand.
Errors During Replication are Corrected by the DNA Polymerase...
6.2K
DNA Replication02:40

DNA Replication

49.3K
DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied.  After replication, each double-stranded DNA includes one parental or “old” strand and one “new” strand. This is known as semiconservative replication. The resulting DNA molecules have the same sequence and are divided equally into the two daughter cells.
Replication in Prokaryotes
DNA replication...
49.3K
Lagging Strand Synthesis01:59

Lagging Strand Synthesis

51.0K
During replication, the complementary strands in double-stranded DNA are synthesized at different rates. Replication first begins on the leading strand. Replication starts later, occurs more slowly, and proceeds discontinuously on the lagging strand.
There are several major differences between synthesis of the leading strand and synthesis of the lagging strand. 1) Leading strand synthesis happens in the direction of replication fork opening, whereas lagging strand synthesis happens in the...
51.0K
Replication in Prokaryotes01:32

Replication in Prokaryotes

24.8K
DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...
24.8K

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相关实验视频

Updated: Jun 23, 2025

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

508

在复制过程中绘制快速DNA聚合酶交换的地图.

Longfu Xu1, Matthew T J Halma1, Gijs J L Wuite2

  • 1Department of Physics and Astronomy and LaserLab, Vrije Universiteit Amsterdam, De Boelelaan 1081, 1081 HV, Amsterdam, The Netherlands.

Nature communications
|June 22, 2024
PubMed
概括
此摘要是机器生成的。

在复制过程中,DNA聚合酶迅速和自主地交换,挑战了蛋白质辅助模型. 这种自主复制模型的特点是:

更多相关视频

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase
07:27

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase

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Visualizing Single-molecule DNA Replication with Fluorescence Microscopy
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Visualizing Single-molecule DNA Replication with Fluorescence Microscopy

Published on: October 9, 2009

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相关实验视频

Last Updated: Jun 23, 2025

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
05:37

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

Published on: April 4, 2025

508
Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase
07:27

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase

Published on: April 29, 2010

13.6K
Visualizing Single-molecule DNA Replication with Fluorescence Microscopy
15:57

Visualizing Single-molecule DNA Replication with Fluorescence Microscopy

Published on: October 9, 2009

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科学领域:

  • 分子生物学分子生物学
  • 生物化学 生化学
  • 遗传学 遗传学 是一个

背景情况:

  • DNA复制机制得到了广泛的研究,但DNA聚合酶交换动态仍然不清楚.
  • 目前的模型表明,像酶这样的蛋白质伙伴在复制过程中促进DNA聚合酶交换.

研究的目的:

  • 为了研究复制过程中DNA聚合酶交换的机制.
  • 为了确定DNA聚合酶交换是蛋白质协调的还是自主性的.

主要方法:

  • 使用了机械DNA操纵技术.
  • 单个光蛋白观测被用来跟踪DNA聚合酶动态.

主要成果:

  • 数据揭示了DNA聚合酶在复制过程中的快速和自主交换,独立于其他蛋白质.
  • DNA聚合酶表现出快速解结和重新结合的动态,偏好聚合酶,外核酶活性或暂停.
  • 观察到一种"记忆效应",DNA聚合酶在重新结合时倾向于保持其以前的活动.

结论:

  • 这些发现支持自主DNA复制模型.
  • 这个模型包含了快速的蛋白质交换,活动爆发,以及用于过程复制的"记忆效应".