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选和选择具有免疫调节和抗克洛斯特里侵蚀性质的ebiotic化合物

Feba Ann John¹, Carissa Gaghan¹, Jundi Liu²

¹Department of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA.

Poultry science

|June 23, 2024

概括

这项研究开发了一个体外模型,使用巨来选禽类肠道健康的ebiotic化合物 (ECs). 单氨酸,绿茶提取物,迷香提取物和甲酸显示出有前途的免疫调节和抗克洛斯特里透效应.

科学领域:

禽畜科学禽畜科学禽畜科学
免疫学免疫学免疫学
微生物学微生物学

背景情况:

益生菌是促进家禽肠道健康的料添加剂.
在体内测试之前,在体内测试前,评估eubiotic化合物 (ECs) 免疫调节和抗菌性质在体内是至关重要的.
杆菌 (Clostridium perfringens) 是家禽中重要的肠道病原体.

研究的目的:

使用巨 (MQ-NCSU) 建立一个体外模型,以选ECs以检测免疫调节和抗Clostridium perfringens活动.
评估10种不同的EC对巨细胞基因表达和蛋白质生产的影响.
确定具有在禽类体内疾病预防潜力的EC.

主要方法:

一个巨细胞系 (MQ-NCSU) 被用来选10个EC.
通过细胞活力测定来确定最佳EC度.
在EC治疗后分析了基因表达 (IFNγ,IL-1β,IL-6,IL-10,TGFβ,CD40) 和MHC-II蛋白质表达.
用选定的ECs预处理的巨被感染了C. perfringens以评估抗菌作用.

主要成果:

单氨酸,甲酸和绿茶提取物调节了免疫基因表达和MHC-II蛋白水平.
迷香提取物和 tartaric 酸也显示显著上调IL-10或TGFβ.
单氨酸,绿茶提取物,迷香提取物和丁酸显著抑制了C. perfringens在体外的生长.

结论:

一种体外鸟类巨模型有效地选了ECs的免疫调节和抗C.perfringens特性.
选择的ECs (单氨酸,绿茶提取物,迷香提取物,丁酸) 显示了对家禽肠道健康和疾病预防的潜力.
该模型有助于选择有前途的ECs进行进一步的体内验证.

关键词:

克洛斯特里 (Clostridium perfringensens) 是一种有毒的植物.抗微生物性质的抗菌性质欧生菌是什么欧生菌是什么免疫调节免疫调节免疫调节禽类禽类禽类禽类

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