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相关概念视频

Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

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Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
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Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

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Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
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Lineage Commitment01:21

Lineage Commitment

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Commitment is the  process whereby stem cells:
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Chromatin Modification in iPS Cells01:32

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Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
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Introduction to Nuclear Reprogramming01:14

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Nuclear reprogramming is the process of switching gene expression of one cell type to that of another cell type, usually from a differentiated cell state to an undifferentiated cell state. Differentiation occurs during processes such as development and morphogenesis, tissue regeneration, and malignancy. Cells can also be artificially induced to reprogram their gene expression by techniques such as nuclear transfer, induced pluripotency, and cell fusion. Such techniques have many applications in...
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Differentiation of Common Myeloid Progenitor Cells01:15

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Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...
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Updated: Jun 23, 2025

Reprograming Model of Human Monocyte-derived Macrophages for In-vitro Assays
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LL37/自我DNA复合体介导单细胞重编程.

Aman Damara1, Joanna Wegner1, Emily R Trzeciak1

  • 1Department of Dermatology, University Medical Center of the Johannes Gutenberg-University of Mainz, Mainz, Germany.

Clinical immunology (Orlando, Fla.)
|June 23, 2024
PubMed
概括
此摘要是机器生成的。

抗微生物LL37和自我DNA触发单细胞的炎症和表观遗传变化,导致像牛皮这样的自身免疫性疾病. 抑制KDM6A/B逆转了这些训练免疫效应,提供了治疗潜力.

关键词:
在LL37/自我DNA复合体中.代谢和表观遗传修饰一个单细胞重编程.牛皮是一种病.

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Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes
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Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes
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科学领域:

  • 免疫学 免疫学 免疫学
  • 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
  • 代谢变化 代谢变化

背景情况:

  • LL37和自我DNA复合体与牛皮和狼等自身免疫性疾病有关.
  • 这些复杂物可以诱导髓状细胞的持久变化,影响免疫反应.

研究的目的:

  • 研究LL37/自我DNA复合体对单细胞的长期代谢和表观遗传影响.
  • 探索KDM6A/B在LL37/自我DNA诱导的训练免疫表型中的作用.

主要方法:

  • 用LL37/自DNA复合体对单细胞的处理.
  • 分析代谢活性 (糖解,氧化酸化) 和细胞因子的释放.
  • 评估KDM6A/B表达和功能抑制.
  • 对原始CD4+T细胞的影响的评估.

主要成果:

  • LL37/自我DNA复合物诱导单细胞中的高代谢率和促炎性细胞因子释放,模仿牛皮单细胞.
  • 在接受治疗的单细胞和牛皮患者的单细胞中,KDM6A/B脱甲基酶的调节升高.
  • 抑制KDM6A/B可以逆转受过训练的免疫表型,减少炎症和T细胞诱导.

结论:

  • LL37/自我DNA诱导的先天免疫记忆有助于牛皮病原.
  • KDM6A/B在调解单细胞中训练免疫反应方面发挥着至关重要的作用.
  • 针对KDM6A/B可能为牛皮和相关的自身免疫性疾病提供治疗策略.