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在捐赠者例行中对D变异的血清分析:揭示对假阴性结果和免疫接种的影响

Carine P Arnoni¹, Tatiane A Vendrame¹, Flavia S Silva¹

¹1Colsan-Associação Beneficente de Coleta de Sangue, São Paulo, SP, Brazil.

Immunohematology

|June 24, 2024

概括

识别D变体对于准确的血型定型和预防非免疫接种至关重要. 这项研究发现,特定的变异,如弱D型38型,会导致假阴性,影响输血安全.

科学领域:

输血医学输血医学
免疫学免疫学免疫学
遗传学遗传学是一种遗传学.

背景情况:

在输血方面,D变体带来了挑战,导致潜在的Rh免疫球蛋白过度使用和抗D合免疫.
D型变异的流行率和血清学行为在各族群之间存在差异,需要定制的测试策略.
了解D变异是优化常规血清学测试和临床实践的关键.

研究的目的:

为了研究D型变异的血清学特征.
为了识别与错误阴性D输入结果最相关的D变体.
为了确定与非免疫接种相关的D变异.

主要方法:

分子测试在供体和患者样本中表征了37种RHD变体.
血清检测包括两个研究期间的半自动和自动平台.
特别选择了不一致的D型或D+与抗D型的样本.

主要成果:

83.4%的样本显示出差异或非典型的反应性;12.3%的样本在捐赠之间有不一致的结果.
DAR1.2是最常见的变种;弱D型38导致75%的不一致样本.
DIVa和DIIIc变异与非免疫接种有关,并未通过标准血清学测试检测到.

结论:

选择检测弱D型38和11的供体查试验至关重要,特别是在多样化的群体中.
输血前的测试需要与DAR变体反应的试剂和策略来识别DIVa和DIIIc.
精确检测D型变种可以提高输血安全性,并降低免疫接种风险.

关键词:

D 打字不一致打字不一致 D 变种 D 变种在 RHD 变体中.这是一种抗D抗体.部分的 D 部分的 D 一个弱小的,一个弱小的.

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