在体外回忆:子宫编程在体内影响各自的光线上皮细胞功能在体外†
Cecilia Constantino Rocha1, Andrey Luiz Lopes Cordeiro2, Meghan Campbell1
1Department of Animal Sciences, University of Florida, Gainesville, Florida, 32611, USA.
Biology of reproduction
|June 24, 2024
概括
牛子宫细胞保留了体内刺激的"细胞记忆",影响它们在培养中的行为. 这一发现为牛的生育能力和子宫内膜反应提供了洞察力.
科学领域:
- 生殖生物学 生殖生物学
- 细胞和分子生物学 细胞和分子生物学
- 动物科学动物科学
背景情况:
- 牛子宫内膜在消化期和怀孕初期对短暂的刺激表现出动态的细胞反应.
- 了解这些刺激如何长期影响子宫内膜细胞对于牛的繁殖成功至关重要.
研究的目的:
- 建立一个模型来研究牛子宫内膜中的细胞记忆.
- 为了调查体内刺激是否被保留在孤立的牛子宫上皮细胞 (BUEC) 中作为细胞记忆.
- 在体外测量BUEC增殖,迁移和对重组牛干扰素tau (rbIFNT) 的反应.
主要方法:
- 从经历雌性期的奶牛中分离出BUEC,培养,并接受各种实验条件 (怀孕状态,孕补充剂,COX-1/2抑制剂治疗).
- 实验室试验包括扩散/迁移 (划痕试验) 和对rbIFNT的反应.
- 对于所有实验,子宫内膜细胞学是在雌激发后4天收集的.
主要成果:
- 来自孕母牛的BUEC显示了增强的rbIFNT响应和扩散.
- 活体内补充孕激素导致抑制BUEC增殖和增加RSAD2表达.
- 弗鲁尼克辛梅格因以IFN信号通路特定的方式改变了BUEC对rbIFNT的响应.
- 不同的奶牛群体在BUEC繁殖能力和rbIFNT响应能力上表现出固有的差异.
结论:
- 体内子宫内膜体验的生理和药理刺激在体内被保留为BUECs的细胞记忆.
- 这种细胞记忆在体外培养过程中持续存在,改变了BUEC的增殖,迁移和rbIFNT的响应性.
- 这些发现提供了关于细胞记忆在牛生育能力和子宫内膜功能中的作用的见解.
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