不同深度RNA测序用于在炎症性心肌病中诊断微生物感染的检测
Weihua Huang1, Changhong Yin1, Patrick A Lento2
1Department of Pathology and Laboratory Medicine, East Carolina University, Greenville, NC (W.H., C.Y., J.T.F.).
Circulation. Genomic and precision medicine
|June 24, 2024
概括
微分元转录组学下一代测序 (mtNGS) 可以识别炎症性心肌病中的微生物感染和宿主反应. 这种方法有助于了解疾病的原因,并指导患者的治疗.
科学领域:
- 心脏病学 心脏病学
- 传染性疾病 传染性疾病
- 基因组学就是基因组学.
背景情况:
- 炎症性心脏病具有不同的传染性和非传染性来源.
- 在炎症性心肌病和微生物感染之间存在潜在的联系.
研究的目的:
- 调查炎症性心肌病与微生物感染有关的假设.
- 开发和验证用于检测心脏组织中的病原体的诊断协议.
主要方法:
- 追溯性研究,利用在甲固定型胺嵌入心脏组织上的深度RNA测序.
- 关于单样和对对照病例的协议开发和可行性测试.
- 不同元转录组学下一代测序 (mtNGS) 的应用,用于病原体和宿主反应分析.
主要成果:
- 不同的mtNGS在心脏组织中发现了潜在的致病微生物 (例如, *Cutibacterium acnes*, *Corynebacterium aurimucosum*, *Pseudomonas denitrificans*)
- 该方法描述了个体宿主免疫反应,包括基因表达改变和推断免疫细胞组成.
- 还确定了有助于感染易感性的遗传变异.
结论:
- 不同的mtNGS有效地捕捉了微生物病原体和宿主在炎症性心肌病的免疫状态.
- 这种方法增强了对疾病病原和个体病因学的理解.
- 开发的方法显示了在诊断和指导炎症性心肌病的治疗中临床翻译的潜力.
更多相关视频
04:41Digital PCR for Quantifying Circulating MicroRNAs in Acute Myocardial Infarction and Cardiovascular Disease
Published on: July 3, 2018
8.1K
05:54Preparation of a Non-Cardiomyocyte Cell Suspension for Single-Cell RNA Sequencing from a Post-Myocardial Infarction Adult Mouse Heart
Published on: February 3, 2023
2.0K
相关概念视频
Myocarditis II: Clinical Features and Diagnostic Tests
Myocarditis is an inflammation of the heart muscle. The symptoms vary widely, encompassing asymptomatic presentations to severe, acute manifestations.Clinical PresentationAsymptomatic cases: In some instances, myocarditis may be asymptomatic, with the infection resolving without intervention. These cases often go undetected unless discovered incidentally through diagnostic imaging or tests conducted for other reasons.General Early Symptoms: Early symptoms of myocarditis are non-specific and can...
Methods of Classification and Identification
Bacterial identification relies on a diverse array of techniques to classify and understand microorganisms, each tailored to uncover specific characteristics. Traditional morphological approaches, while still valuable, are limited for closely related or structurally simple organisms. Modern methods integrate biochemical, serological, genetic, and advanced molecular tools to achieve greater accuracy.Morphological and Biochemical TechniquesMorphological characteristics, such as cell shape and...
Modern Molecular Taxonomy
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
Automated Microbial Diagnostics
Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...
