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Updated: Jun 23, 2025

Cell Co-culture Patterning Using Aqueous Two-phase Systems
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Cell Co-culture Patterning Using Aqueous Two-phase Systems

Published on: March 26, 2013

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通过水性三相分区系统分离细胞外聚合物质

Evelyn C Antunes1,2, Bruna Cintra1, Matthieu Bredel1

  • 1Wetsus-European Centre of Excellence for Sustainable Water Technology, Oostergoweg 9, 8911MA Leeuwarden, The Netherlands.

Industrial & engineering chemistry research
|June 24, 2024
PubMed
概括

这项研究展示了一种具有成本效益的三相分区 (TPP) 系统,用于分离微生物细胞外聚合物质 (EPS). TPP方法有效地分成EPS组件,显示出循环经济应用的潜力.

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Subcellular Fractionation01:32

Subcellular Fractionation

The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...
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Extraction: Partition and Distribution Coefficients01:14

Extraction: Partition and Distribution Coefficients

The distribution law or Nernst's distribution law is the law that governs the distribution of a solute between two immiscible solvents. This law, also known as the partition law, states that if a solute is added to the mixture of two immiscible solvents at a constant temperature, the solute is distributed between the two solvents in such a way that the ratio of solute concentrations in the solvents remains constant at equilibrium.
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Ion Exchange01:17

Ion Exchange

Ion exchange chromatography separates charged molecules from a solution by reversibly exchanging them with mobile, or 'active', ions associated with the oppositely charged stationary phase. This method can be used to separate ions, soften and deionize water, and purify solutions. The polymers comprising the ion-exchange column are high-molecular-weight and chemically stable polymers, crosslinked to be porous and essentially insoluble. They are also functionalized with either acidic or...
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Extraction: Advanced Methods00:56

Extraction: Advanced Methods

Metal ions can be separated from one another by complexation with organic ligands–the chelating agent– to form uncharged chelates. Here, the chelating agent must contain hydrophobic groups and behave as a weak acid, losing a proton to bind with the metal. Since most organic ligands used in this process are insoluble or undergo oxidation in the aqueous phase, the chelating agent is initially added to the organic phase and extracted into the aqueous phase. The metal-ligand complex is...
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