替代性索拉尼 (Alternaria solani) 作用因子AsCEP19和AsCEP20揭示了在致病性和结合性产生方面的新功能
Siyu Xiao1, Jinhui Wang1,2, Zihan Bai1
1College of Plant Protection, Hebei Agricultural University, Baoding, Hebei, China.
Microbiology spectrum
|June 24, 2024
概括
该研究表明,Alternaria solani效应器AsCEP19和AsCEP20对于真菌发育和病原性至关重要. 删除这些基因会损害状植物的成熟,并显著减少宿主植物的毒性.
科学领域:
- 植物病理学 植物病理学
- 分子菌学 分子菌学.
- 菌类遗传学 菌类遗传学
背景情况:
- 阿尔特纳里亚索拉尼 (Alternaria solani) 导致早期腐烂,这是土豆和西红作物的重要疾病.
- 具体的作用因子AsCEP19和AsCEP20以前与A. solani.病毒性有关.
- 在A. solani感染中,AsCEP19和AsCEP20的确切作用和机制尚未完全理解.
研究的目的:
- 为了研究AsCEP19和AsCEP20在Alternaria solani中的功能.
- 确定AsCEP19和AsCEP20对真菌发育,病原性和宿主-病原体相互作用的影响.
- 阐明AsCEP19和AsCEP20的亚细胞定位和潜在宿主点.
主要方法:
- 在A. solani.中构建和分析AsCEP19和AsCEP20删除突变.
- 评估菌生长,菌成熟和与菌发生相关的基因表达.
- 在土豆和番茄植物上进行致病性测定.
- 使用GFP标记蛋白的亚细胞局部化研究.
- 权重基因共同表达网络分析.
主要成果:
- 删除突变 (ΔAsCEP19, ΔAsCEP20) 显示出正常的植物生长,但受损的状体成熟,异常状体增加.
- 在突变菌株中,调节基因abaA和chsA在基生成中的表达发生了变化.
- 突变菌株在土豆和番茄植物上显著降低了毒性.
- AsCEP19局限于叶绿体,而AsCEP20局限于叶绿体和宿主细胞中的血.
- 基因共同表达分析表明,这些效应因子与光合作用途径之间存在联系.
结论:
- AsCEP19和AsCEP20在A. solani.的状发育和成熟中起着至关重要的作用.
- 这些效应因子对于A. solani在宿主植物上的致病性至关重要.
- AsCEP19和AsCEP20可能向叶绿体,并影响光合作用,导致毒性.
关键词:
另一个是Alternaria solani.在AsCEP19和AsCEP20中.这就是基生成 (conidiogenesis).病原性是一种病原性.亚细胞局部化的局部化权重基因共同表达网络分析.更多相关视频
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