相关实验视频

Updated: Jun 23, 2025

Quantitative Multispectral Analysis Following Fluorescent Tissue Transplant for Visualization of Cell Origins, Types, and Interactions

Quantitative Multispectral Analysis Following Fluorescent Tissue Transplant for Visualization of Cell Origins, Types, and Interactions

Published on: September 22, 2013

多目标光在现场混合诊断应用在瘤中的应用

Martina Amato¹, Jeremy A Squire², Renato Franco¹

¹Department of Mental and Physic Health and Preventive Medicine, Pathology Unit, University of Campania Luigi Vanvitelli, Naples, Italy.

Methods in molecular biology (Clifton, N.J.)

|June 24, 2024

概括

多目标光在位杂交 (mFISH) 检测癌症组织中的多个遗传点. 这种测定对于在甲固定,嵌样本中识别生物标志物是有价值的.

科学领域:

分子生物学分子生物学
在瘤学瘤学.
遗传学是一个遗传学.

背景情况:

多目标光 in situ 杂交 (mFISH) 能够同时检测多个核酸序列.
在多重分析中,mFISH使用了光谱上独特的光标签.
该技术在瘤学中越来越多地用于生物标志物识别.

研究的目的:

总结mFISH在识别各种瘤类型中的遗传异常方面的应用.
讨论mFISH协议,特别针对甲固定,嵌 (FFPE) 组织样本.
在mFISH分析中突出创新的多目标探测和关键解释问题.

主要方法:

应用mFISH技术在甲固定,嵌 (FFPE) 组织样本上.
使用光谱上不同的光体标签,同时检测多个目标序列.
开发和应用创新的多目标探测器.

主要成果:

在不同类型的瘤中,mFISH成功地在FFPE样本中识别了瘤基因异常.
已建立的协议有助于在FPE组织中进行mFISH分析.
证明了多目标探针对于综合基因分析的实用性.

结论:

mFISH是用于在瘤学中检测预测性,预后性和诊断性生物标志物的强大工具.
讨论的协议和探针增强了mFISH在FFPE样本中的应用.
仔细解释mFISH结果对于准确的临床应用至关重要.

关键词:

诊断生物标志物诊断生物标志物用甲固定,嵌入胺的组织样本.多目标光在现场混合化.预测性生物标志物预测性生物标志物瘤这些瘤

更多相关视频

A Rapid Method for Multispectral Fluorescence Imaging of Frozen Tissue Sections

A Rapid Method for Multispectral Fluorescence Imaging of Frozen Tissue Sections

Published on: March 30, 2020

Multiplexed Immunofluorescence Analysis and Quantification of Intratumoral PD-1+ Tim-3+ CD8+ T Cells

Multiplexed Immunofluorescence Analysis and Quantification of Intratumoral PD-1+ Tim-3+ CD8+ T Cells

Published on: February 8, 2018

相关概念视频

FISH - Fluorescent In-situ Hybridization

FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...

In-situ Hybridization

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...