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YY1/circCTNNB1/miR-186-5p/YY1正循环加剧了通过Wnt途径的肺癌进展

Yanli Shen¹, Yan Yang¹, Yan Zhao¹

¹Department of Pulmonary Medicine, The Affiliated Cancer Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China.

|June 24, 2024

概括

循环RNA circCTNNB1通过激活Wnt通路并与YY1和miR-186-5p形成正反循环,促进肺癌的进展,导致预后不佳.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
癌症研究癌症研究

背景情况:

肺癌仍然是一个重大的全球健康挑战.
循环RNAs (circRNAs) 正在成为各种疾病的关键调节者.
circCTNNB1在肺癌病原发生中的特定作用在很大程度上是未知的.

研究的目的:

阐明肺癌中circCTNNB1的功能和调节机制.
研究circCTNNB1通路内的潜在治疗点.

主要方法:

实时聚合酶连锁反应 (RT-qPCR) 和西部黑斑用于表达分析.
细胞增殖,迁移,入侵和衰老的测试 (CCK-8,Transwell,SA-β-gal).
露西法酶记者和RNA免疫沉降 (RIP) 测试以确认分子相互作用.
在体内瘤生长分析.

主要成果:

肺癌组织中circCTNNB1的表达上调,并与预后不佳有关.
circCTNNB1促进肺癌细胞的增殖,迁移和入侵,同时抑制衰老.
circCTNNB1通过海绵化miR-186-5p准YY1,YY1积极调节circCTNNB1转录,形成一个YY1/circCTNNB1/miR-186-5p/YY1循环.
这个循环调节Wnt信号通路,并加速瘤生长.

结论:

涉及YY1,circCTNNB1和miR-186-5p的积极反循环驱动肺癌的进展.
在肺癌中,circCTNNB1通过调节Wnt路径,起到瘤原因驱动的作用.
针对这个循环可能为肺癌提供一种新的治疗策略.

关键词:

电路电路电路NB1 没有路径的路径.YY1 YY1 这是一个很棒的节目.肺癌是一种肺癌.在 miR-186-5p 中.

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