微波辅助生物基生物气改造和生物糖酸生产的多目标优化
Amir Izzuddin Adnan1, Mei Yin Ong1, Hassan Mohamed1
1Institute of Sustainable Energy, Universiti Tenaga Nasional, 43000 Kajang, Selangor, Malaysia.
Bioresource technology
|June 24, 2024
概括
这项研究引入了一种新的方法,用于生产生物酸 (bio-SA) 和使用共同培养的细菌, ragi tapai 和宏观藻类的丰富生物甲. 微波预处理优化了这些同时进行的生物过程,为二氧化碳捕获提供了潜在的成本节约.
科学领域:
- 生物技术是生物技术.
- 生物化学工程 生物化学工程
- 环境科学 环境科学
背景情况:
- 开发生物酸 (bio-SA) 和生物甲生产的可持续方法对于循环经济至关重要.
- 二氧化碳 (CO2) 的捕获和利用仍然是工业过程中的重大挑战.
- 与藻类共同培养微生物为增强生物过程提供了一个有希望的途径.
研究的目的:
- 通过共同培养系统研究生物酸 (bio-SA) 和甲丰富的联合生产.
- 评估微波辐射作为预处理方法对 ragi tapai 的影响,以增强生物化学过程.
- 优化关键参数,包括微波辐射剂量,宏藻比率和pH值,以最大限度地提高生物SA和生物甲产量.
主要方法:
- 使用 ragi tapai 和 Chaetomorpha 大藻共同培养,同时生产生物SA 和生物甲.
- 应用微波辐射作为 ragi tapai 的预处理,以增强微生物活动.
- 使用Design-Expert软件来确定微波剂量,Chaetomorpha比率和pH的最佳条件.
主要成果:
- 实现了85.7%的最佳生物甲丰富度和0.65g/L的生物糖酸 (bio-SA) 生产.
- 确定最佳条件为微波剂量为1.45 W/g,Chaetomorpha比为0.9,pH为7.8.
- 证明了微波辐射在促进同时缩甲和生物SA生产方面的有效性.
结论:
- 在共培系统中,微波预处理的 ragi tapai 有效地增强了同时生产的生物SA和生物甲.
- 优化条件为具有成本效益的二氧化碳捕获和生物气改造提供了途径.
- 这种综合方法为废物利用和可再生能源生产提供了可持续的解决方案.
相关概念视频
Environmental Applications of Microorganisms
Microorganisms play a pivotal role in maintaining ecosystem balance by recycling essential elements such as carbon, nitrogen, and phosphorus, as well as supporting processes like bioremediation, wastewater treatment, and biofuel production.Microbes in Elemental CyclesIn the carbon cycle, microorganisms decompose organic matter, releasing carbon dioxide via aerobic respiration. This carbon dioxide is subsequently used by photosynthetic organisms to synthesize organic compounds, closing the...
Bioreactor Design and Operational System
Bioreactors are engineered vessels designed to cultivate microorganisms under controlled conditions for industrial bioprocessing. They maintain sterility and allow precise regulation of pH, temperature, oxygen, and nutrient levels to optimize microbial growth and metabolite production. Bioreactors range from small laboratory units of 1 liter to industrial systems holding up to 500,000 liters, though only about 75% of their volume is actively used for fermentation. The remaining headspace...
Bioreactor Controls-III
Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
Methods of Medium Optimization
Optimizing growth media enhances microbial proliferation and maximizes product yield. Statistical experimental design methodologies provide structured and reproducible approaches, offering progressively higher levels of robustness and efficiency.The One-Factor-at-a-Time (OFAT) MethodThe One-Factor-at-a-Time (OFAT) method involves adjusting a single variable while keeping all others constant. However, it cannot detect interactions between variables, often leading to suboptimal outcomes when...
Production of Biopesticides
Biopesticides offer a sustainable alternative to chemical pesticides, utilizing microbial agents to control agricultural pests. Bacillus thuringiensis (Bt) is a widely employed bacterium known for its potent insecticidal activity. Bt biopesticides are favored for their specificity to insect pests, minimal environmental impact, and natural degradability.Mechanism of Bt Toxin Action Bt produces insecticidal crystal (Cry) proteins during its sporulation phase. These proteins form parasporal...
Biofuels
The microbial conversion of organic matter into biofuels holds potential as a renewable energy source. Among biofuel sources, microalgae are recognized as a highly efficient and adaptable feedstock for biodiesel production, owing to their rapid biomass accumulation, elevated lipid productivity, and capacity to proliferate in diverse aquatic systems, including freshwater, marine, and wastewater habitats. Unlike terrestrial crops, microalgae do not compete for land and can achieve significantly...


